| 【Objective】The natural propagation mode for Murraya paniculata is sowing, which can not meet the
needs of extensive production of plantlets. The establishment of efficient in vitro regeneration system lays a foundation
for industrialized breeding of tissue culture seedlings for M. paniculata.【Method】Mature seeds of M. paniculata were
used as explants to study the effects of different concentrations and ratios of hormone and different organic matters on seed
germination, proliferation, strong seedling culture and rooting culture of M. paniculata. Then, sterile tissue culture seedlings
were obtained for transplanting.【Result】After the pretreatment with 75% alcohol and 0.1% HgCl2
, the most suitable
medium for seeds germination is MS+6-BA 0.5 mg/L, and the germination rate is 62.50%; the most appropriate basic
medium for subculture proliferation of cluster buds is the MS medium; the most suitable medium for multiplication culture is MS + 6-BA 0.50 mg/L + NAA 0.15 mg/L, and the multiplication coefficient is up to 2.37; the suitable medium for strong
seedling culture is MS + potato juice 30 g/L, and the plant height increase to 2.0 cm in 40 days; the optimal medium for rooting
culture is 1/2MS + IBA 0.6 mg/L + potato juice 30 g/L+ banana juice 30 g/L + AC 0.1 mg/L, and the rooting rate is 55.6%.
The survival rate of the rooting seedlings is 85% after domesticated and transplanted into the mixed matrix (peat soil: loess:
perlite: fertilizer of calcium, magnesium and phosphate, 3∶2∶1∶0.2).【Conclusion】Different hormones and concentrations have
significant effects on seed germination and cluster bud proliferation. Adding potato juice and banana juice are beneficial to the
growth of plantlets and rooting. |
