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| Genetic Analysis and QTL Mapping ofPericarp Thickness in Sweet Corn |
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| DOI：10.16768/j.issn.1004-874X.2022.08.003 |
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| Abstract: |
| 【Objective】In order to study the genetic mechanism of sweet corn pericarp thickness and select highquality sweet corn varieties, the major genes and polygenes genetic analysis and QTL mapping of sweet corn pericarp
thickness were carried out.【Method】The sweet corn inbred lines T15 and T77 with large differences in pericarp thickness
were selected to prepare hybrid combination (T77×T1). The F2
population of the combination was used as the experimental
material, the main genes and polygenes hybrid genetic method was used to analyze relevant parameters of the genetic model;
Combined with the pericarp thickness and SSR genetic linkage map of each individual plant in F2
population, QTL mapping
of pericarp thickness in sweet corn was carried out by using composite interval mapping method.【Result】It was found that
A-1 was the optimal model for sweet corn pericarp thickness, that is, an additive and partially dominant genetic model
controlled by a pair of major genes, and the heritability of major genes was 69.10%. There were three QTLs identified on chromosomes 5 and 8 respectively, which were related to pericarp thickness. Two QTLs were detected in region bin 5.04 of
chromosome 5, which were located in the marker interval bnlg150-bnlg653 and bnlg653-bnlg1208 respectively, and the
additive effect values were -2.39 and -3.01 respectively. The QTL located on chromosome 8 was in bin8.03-bin8.04, the
marker interval was umc1741-bnlg2046, the additive effect value was -3.06, and the contribution rate to phenotype was
22.02%.【Conclusion】The pericarp thickness of sweet corn is dominated by the effect of major genes. In breeding practice,
genetic improvement and selection can be carried out in the early generation. The QTLs detected in this experiment can be used
for molecular marker assisted selection and quality breeding. |
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