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| Identification of Peanut Pod Rot Pathogens in GuangdongProvince and Screening of Fungicides |
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| DOI：10.16768/j.issn.1004-874X.2022.08.009 |
| Author Name | Affiliation | | XING Juejun1
, XU Yuzhao2,3, SU Yutong2,3, WEI Xinying2,4, JIANG Haiqi2,3, YU Guohui2,3, YIN Yan5 | 1. 仲恺农业工程学院实验与设备处，广东 广州 510225；
2. 仲恺农业工程学院植物健康创新研究院 / 农业农村部华南果蔬绿色防控重点实验室 /
广东省普通高校果蔬病虫害绿色防控重点实验室，广东 广州 510225；
3. 仲恺农业工程学院农业与生物学院，广东 广州 510225；
4. 仲恺农业工程学院资源与环境学院，广东 广州 510225；
5. 广东省农业科学院蔬菜研究所，广东 广州 510640 |
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| Abstract: |
| 【Objective】The study was conducted to identify the pathogenic types of peanut pot rot in Guangdong
and screen effective fungicides in order to provide a reference for the prevention and control of the disease in production.
【Method】The pure fungal cultures of fruit rot were isolated from the diseased peanut pods collected in fields by tissue
isolation method, the pathogens were confirmed after the pathogenicity of the isolated strains were verified by back inoculation,
and the pathogenic types were identified by cloning the strain’s ITS sequence and phylogenetic analysis. The tissue samples
were used to isolate fungi from rotted peanut pods. The pathogenicity of all isolates were tested based on Koch’s rule, and then
identified by ITS sequences analysis. The inhibition effect of 40% thiram WS, 98% hymexazol SP, 24% Jinggangmycin A AS,
10% difenoconazole WDG and 10% polyoxins WP against the peanut pod rot pathogens were determined by mycelia growth rate
method. The EC50 values of different fungicides were analysed.【Result】Two pathogenic fungi were isolated from the peanut
pod rot samples collected in Shaoguan. Both of them could cause peanut pod rot and even kernel rot after back inoculation.
Phylogenetic analysis based on ITS sequences showed that the pathogens belonged to Fusarium oxyspourm and Fusarium
solani. The inhibitory effect of 40% thiram WS was higher than other fungicides, and the EC50 value on F. oxyspourm and
F. solani were 0.001 mg/L and 0.021 mg/L, respectively. The EC50 value of 98% hymexazol SP on two Fusairum strains were
0.296 mg/L and 0.217 mg/L, respectively. The EC50 value of 24% Jinggangmycin A AS on two Fusairum strains were 20.575 mg/L
and 11.185 mg/L, respectively. 10% difenoconazole WDG and 10% polyoxins WP had no inhibitory effect on the two Fusarium
pathogen strains.【Conclusion】Peanut pod rot in Guangdon is mainly caused by Fusarium co-infection, and F. solani is the
main pathogen. The two strains F. oxyspourm and F. solani are both sensitive to thiram and hymexazol, which can be used as
fungicides for the prevention in production. |
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