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| Effects of Feeding Frequency, Water Temperature and BreedingDensity on Growth Perfirmanc e, Intestinal Structure and HepaticGrowth-related Gene Expression Hepatic of Largemouth Bass |
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| DOI：10.16768/j.issn.1004-874X.2022.08.014 |
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| Abstract: |
| 【Objective】This experiment was conducted to research the effects of feeding frequency, water temperature
and breeding density on growth performance, intestinal structure and hepatic GH and IGF-1 gene expression levels of
largemouth bass in an indoor recirculating aquaculture system so as to determine the appropriate feeding frequency, water
temperature and breeding density of largemouth bass.【Method】The experiment consisted of 8 treatments (Ⅰ - Ⅷ ). After 8
weeks of breeding, the growth performance indicators such as specific growth rate (SGR), feeding rate (FR), feed coefficient (FCR)
and survival rate, the intestinal villus height, villus width and muscle thickness, and the expression of hepatic growth factor GH
and IGF-1 genes of largemouth bass were evaluated.【Result】Only the main effects of breeding density on FR and feeding
frequency on condition factor (CF) were significant, while the interaction effects had a significant effect on CF. The one-way
ANOVA results showed that, the growth performance of treatment Ⅴ was significantly better than that of other treatments, and
the SGR was significantly higher than that of treatments Ⅱ , Ⅳ , Ⅵ and Ⅷ , increasing by 10.16%, 10.61%, 9.27% and 8.4%,
respectively; the FR was significantly higher than that of treatments Ⅰ , Ⅱ , Ⅳ and Ⅵ , increased by 10.94%, 27.02%, 26.45%
and 17.38%, respectively; the FCR was significantly lower than that of treatments Ⅳ , Ⅷ and Ⅷ , decreasing by 7.87%, 8.89%
and 8.89%, respectively. Compared with the treatmentⅤ, the FR of treatment I was significantly lower than that of treatmentⅤ,
but there were no significant differences in SGR and FCR. The main effect and the interaction effect on intestinal villus height,
villus width, muscle thickness and hepatic GH and IGF-1 gene expression levels were not significant. The intestinal villus
height of treatment Ⅴ was significantly higher than that of treatments Ⅳ , Ⅵ and Ⅶ , villus width of treatments Ⅰ and Ⅴ was
significantly higher than that of treatments Ⅲ , the intestinal muscle thickness of treatments Ⅰ and Ⅲ was thicker than that of
treatment Ⅷ , and there was no significant difference in other treatments. The hepatic GH and IGF-1 gene expression levels of
treatments Ⅰ and Ⅴ were significantly higher than those of other treatments.【Conclusion】The optimal feeding frequency
for largemouth bass was 2-3 times/d, the water temperature was 25 ℃, and the breeding density was 25 fish per tank (150 L). |
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