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| Analysis on Colonization Dynamics and Phosphate Solubilization Effects of a Penicillium Strain in Soil |
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| DOI：10.16768/j.issn.1004-874X.2022.10.009 |
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| Abstract: |
| 【Objective】The colonization dynamics and phosphate solubilization effects of Penicillium brocae in soil
were studied in order to provide references for the development and application of soil phosphate solubilization bioinoculant,.
【Method】A P. brocae transformant Z3 with multiple molecular screening makers was used for soil cultivation test.
Combining with the screening makers, the colony forming units (CFU) of Z3 in soil were quantified with plate counting
method at different time nodes and the soil available phosphate (AP) was measured at the same time.【Result】The green
fluorescent protein (gfp), hygromycin B phosphotransferase (hph) and β-glucuronidase (gus) genes could expressed normally
in Z3, and there was no significant difference in the growth curve and phosphate solubilization ability between Z3 and wild type (Wt) strain. The colonization number of Z3 in soil decreased from 1×106
CFU/g to 1×105
CFU/g at the first stage, then it
increased to the maximum value of 4.3 ×106
CFU/g at the third week and followed by a gradually decreasing to 1.4×103
CFU/g
in soil at the tenth week. The soil AP content showed a trend of increasing first and then decreasing. At the sixth week, the soil
AP reached the maximum value of 14.7 mg/L which increased by 41.3% compared to the AP of original soil. After inoculation for
seven weeks, the soli AP gradually decreased to 12.7 mg/L at the tenth week which still increased by 22.1% compared to the AP
of original soil. Additionally, it was also found that the colonization number of Z3 in soil were close to or higher than 1×105
CFU/g
during the increasing of soil AP and the AP gradually decreased when the number of Z3 was significantly lower than 1×105
CFU/g.
【Conclusion】The transformant Z3 can well colonize for ten weeks after being inoculated into soil, which shows good
colonization ability and increases soil AP concentration significantly. Additionally, the colonization number of Z3 has a positive
role in the maintenance of phosphate solubilization effects. |
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