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| Screening of Lactobacillus Fermentum from Pigletsand Its Potential Probiotic Properties |
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| DOI：10.16768/j.issn.1004-874X.2022.11.006 |
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| Abstract: |
| 【Objective 】A strain of Lactobacillus fermentum was isolated from the feces of healthy piglets, and its
probiotic properties were tested in order to provide references for its applications in livestock and poultry breeding.【Method】
106 strains of lactic acid bacteria were isolated by using Rogosa SL medium, and one strain which was potential for feeding
was preliminarily screened by gram staining, acid and bile salt tolerance tests. The probiotic ability of strain LF1, which was
identified as L. fermentum LF1 by 16S rDNA sequencing, was evaluated, including its antagonistic activity (Escherichia
coli K88, Staphylococcus aureus, Streptococcus suis, Salmonella, and Vibrio, etc.), tolerance to the stimulated acidic
environment in stomach and bile acid, and adhesion to small intestinal epithelial cells.【Result】L. fermentum LF1 had strong tolerance to acid and bile salt, showing its strong probiotic potential. However, the LF1 strain showed low adhesion to pig
intestinal epithelial cells. In terms of antagonism, the culture supernatant of L. fermentum LF1 inhibited the growth of many
animal pathogens significantly.【Conclusion】L. fermentum LF1 had a good probiotics ability. After further experimental
evaluation on animals, it can be used as a probiotic candidate strain for livestock and poultry breeding industry. |
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