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| Genome-wide Identification and Expression Analysis ofAUX/IAA Gene Family Members in Vigna angularis |
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| DOI：10.16768/j.issn.1004-874X.2022.11.018 |
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| Abstract: |
| 【Objective】Auxin is an essential plant hormone, and AUX/IAA is an important auxin initial response
factor that plays a crucial role in the growth and development of plants under environmental stress. To understand the
biological function of VaIAAs in the growth and development of Vigna angularis, genome-wide of VaIAAs gene family
was identified and analyzed.【Method】Bioinformatics was used to identify and analyze genome-wide of AUX/IAA in
Vigna angularis. Expression patterns of VaAUX/IAA gene family in various tissues were analyzed based on RNA-seq.
【Result】A total of 41 VaIAAs genes were identified in genome-wide of V. angularis, and subcellular localization showed
that all of them were localized to the nucleus. Phylogenetic analysis revealed that AUX/IAA proteins from V. angularis,Arabidopsis thaliana, and Oryza sativa clustered into six groups. Gene structure analysis showed that all the genes contained 1-8
introns and VaAUX/IAA genes contained 1-21 exons. Analysis of cis-acting elements found that cis-acting elements involved
in response to biological/abiotic stress were the most, including the most commonly recognized ATTATA.bos, G-box and sp1
motifs. The enrichment of anaerobic-induced response element ARE and plant stress resistance-related MYB elements was
also found. Protein-protein interaction network analysis showed that all VaIAAs were linked to ARF, in addition, some genes
interacting with AXR3 (auxin-induced repressor), MP (transcription factor B3 family protein), and etc. VaIAAs were tissuespecific, with large differences in gene expression levels in different tissues.【Conclusion】The results of bioinformatics
analysis of gene structure, phylogeny, and protein-protein network interactions of the V. angularis AUX/IAA gene family will
provide important clues to reveal the functions of AUX/IAA gene family in the growth and development of V. angularis. |
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