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| Identification and Virulence Assay of the Entomogenous Fungus FE-1 Strain Isolated from Phenacoccus solenoposis |
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| DOI：10.16768/j.issn.1004-874X.2023.06.013 |
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| Abstract: |
| 【Objective】This study aimed to evaluate the biocontrol potential of an entomogenous fungus strain
isolated from Phenacoccus solenoposis, for providing the basis of its development as a biocontrol agent.【Method】
The strain was isolated and purified from the diseased P.solenoposis using tissue isolation method. Based on the cultural
traits, morphological traits and phylogenetic tree analysis, its taxonomic ststus was clarified. The infection mode of strain
on P.solenoposis was explored by scanning electron microscope，which clarified its ability to infect P.solenoposis. The
pathogenicity of the strain to P.solenoposis was measured by leaf dipping and insect dipping method. 【Result】A strain
FE-1 was isolated and purified from the diseased P.solenoposis, the morphological traits results showed that the colony
diameter of strain FE-1 ranged from 50 to 60 mm at 5 days on PDA medium , and the colony was orange-white in front. The conidia morphology had two types, which small type was long column shape and large spore was sickle shape. The strain FE-1
was identified as Fusarium equiseti based on cultural traits, morphological traits and phglogenetic tree analysis. The strain
FE-1 infected into the insect body cavity of P.solenoposis mainly by conidia germination forming germ tubes at the epidermis
for growing horizontally, and the formation of expanded appressorium at the specific contact epidermis, which clarified its ability
to infect P.solenoposis. The results of indoor toxicity measuremerts showed that the pathogenicity of strain FE-1 against female
adults of P.solenoposis was gradually increased with the concentration of conidial suspension. At the spore concentration of
1×108
CFU / mL, LT50 was 3.32 days, while at 7 days after inoculation, the adjusted mortality of P.solenoposis female adults
was 87.50±1.79%, LC50 was 1.8×105
CFU /mL. 【Conclusion】A strain FE-1 isolated and purified from the diseased
P.solenoposis can form expanded appressorium at the specific contact epidermis for infection, which clarifies its ability to infect
P.solenoposis. Meanwhile, the strain FE-1 is highly pathogenic against P.solenoposis, which has great potential for biocontrol
and provide original material for the biocontrol research of P.solenoposis. |
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