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| Analysis of Genetic Diversity of 24 Morinda offi cinalis How. Based on Phenotypic and Molecular Markers |
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| DOI：10.16768/j.issn.1004-874X.2024.05.005 |
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| Abstract: |
| 【Objective】Deqing County, Guangdong Province is a native habitat of Morinda offi cinalis How., a genuine
medicinal herb in China. In the study, the genetic diversity of germplasm resources of 24 varieties of Morinda offi cinalis How.
were analyzed.【Method】A total of 24 Morinda offi cinalis How. resources collected from Deqing County as research objects,
and principal component analysis and molecular marker methods (SRAP and CDD) were used to analyze the genetic diversity
of 24 Morinda offi cinalis How. materials.【Result】Phenotypic analysis showed that, in the cluster map based on principal
component analysis, Guanwei Town, Gaoliang Town, Fengcun Town and Gaoliang Town were clustered in four groups. In
addition,100 and 98 total bands were obtained in 18 pairs of SRAP primer combinations and 16 CDDP primers, among them,there were 50 SRAP polymorphic bands, resulting in a polymorphism ratio of 50%. The average genetic similarity coefficient (GS)
was 0.87, and the GS ranged from 0.71 to 0.97. There were 80 polymorphic bands in CDDP, with a polymorphism ratio of 79.0%.
The GS among germplasm resources ranged from 0.71 to 0.96, with an average (GS) of 0.86. When the coefficient was 0.82, both
SRAP and CDDP molecular markers showed certain similarities among the germplasm resources of Gaoliang Town, Guanxu
Town and Fengcun Town.【Conclusion】Combined with the clustering results of phenotypic and molecular marker, the genetic
relationship is closest between Gaoliang Town and Guanwei Town as well as Gaoliang Town and Fengcun Town. The results of
SRAP and CDDP molecular marker methods have certain differences in the genetic diversity of Morinda offi cinalis How., and
the combined analysis can better reflect the genetic diversity of Morinda offi cinalis How. and the phylogenetic relationship of
Morinda offi cinalis How. resources. |
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