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| Identification, Expression Analysis and Cloning of CaSULTR Gene Family in Pepper |
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| DOI：10.16768/j.issn.1004-874X.2024.06.006 |
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| Abstract: |
| Abstract: 【Objective】As one of the four major nutrients required by plants, sulfur is a crucial for plant growth and
development, playing a pivotal role in enhancing plant resistance against both biotic and abiotic stresses. Sulfate transporters
(SULTR) serve as primary regulators in the absorption and transport of sulfate in plants, playing an irreplaceable role in this
process. The understanding of the fundamental characteristics and expression patterns of CaSULTR genes could help to provide
theoretical support for advancing researches on SULTR in peppers.【Method】Based on the pepper genome, members of the pepper CaSULTR gene family were identified at the whole-genome level. A comprehensive analysis of their physicochemical
properties, conserved Motifs and gene structures was conducted. By utilizing publicly available transcriptome data and qPCR
technology, the expression patterns of CaSULTR genes under various hormone stress conditions were analyzed. Additionally,
differentially expressed genes were cloned for further investigation.【Result】A total of 13 CaSULTR genes were identified
and designated as CaSULTR1;1~1;3, CaSULTR2;1~2;2, CaSULTR3;1~3;7 and CaSULTR4;1. These genes were distributed
on seven chromosomes in pepper, with amino acid lengths ranging from 641 to 947 aa, molecular weights from 69.65 to 104.36
kD, and isoelectric points between 8.42 to 9.62. Characterized by the hydrophobicity, CaSULTR proteins were classified into
four subgroups (Ⅰ-Ⅳ), with subgroup Ⅲ containing the highest number of members. Within the same subgroup, members
exhibited relatively small differences in physicochemical properties, and Motifs and gene structures were relatively conserved.
With the exception of CaSULTR2;1 and CaSULTR2;2, all members possessed Motif 5. Tissue expression analysis indicated that
CaSULTR genes may be involved in the development of organs such as leaves, fruits and flowers, with CaSULTR4;1 exhibiting
expression in leaves, roots and stems. Expression profile analysis under hormone stress, coupled with qPCR results, revealed
significant up-regulation of CaSULTR1;1 and CaSULTR3;4 under hormone induction.【Conclusion】The expression patterns
of CaSULTR genes under hormone stress are identified, suggesting that of CaSULTR1;1 and CaSULTR3;4 may be involved in
the regulation of sulfur metabolism by exogenous hormones. |
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