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| Isolation, Identification, Virulence and Drug Resistance Genes Analysis of Aeromonas veronii from Micropterus salmoides |
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| DOI：10.16768/j.issn.1004-874X.2024.07.006 |
| Author Name | Affiliation | | HE Xuewen1,2, ZHAO Haohang1,2, WEN Yiming1,2, XIA Hongli2
, YU Dapeng1,2, XIA Liqun1,2, LU Yishan1,2 | （1. 广东海洋大学水产学院 / 广东省水产动物病害防控与健康养殖重点实验室 / 广东省水产经济动物
病害控制重点实验室，广东 湛江 524088；2. 广东海洋大学深圳研究院，广东 深圳 518120） |
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| Abstract: |
| 【Objective】The causative agent was isolated from the diseased Micropterus salmoides tissue. The
physiological properties of the pathogenic bacteria were identified, and their virulence genes and drug resistance genes were
analyzed, with an aim to provide guidance for the scientific use of drugs in clinical practice.【Method】Samples were collected
from pathologic tissues of diseased M. salmoides from a fish breeding factory in Zhanjiang, Guangdong Province, China. The bacteria were isolated by dilution coating method, the 16S rRNA sequence comparison and biochemical tests were performed
to identify the species, and the virulence genes, drug resistance genes and drug sensitivity characteristics were also identified.
【Result】Bacteria with round colony morphology, smooth milky white surface and opaque color were isolated from pathologic
tissues of M. salmoides, and numbered as MSB-2, which was identified as Aeromonas in Gram-negative bacteria through
phenotypic identification by Gram staining and physiological and biochemical reactions. By amplifying the 16S rRNA gene
sequence and constructing a phylogenetic tree, the bacterium was further identified as A. veronii. The strain had virulencerelated genes such as act, aer and aexT and resistance-related genes such as tetC, tetA and sull. The results of the drug
sensitivity test showed that the bacterium was sensitive to carbenicillin, doxycycline, cefalexin, neomycin, gentamicin, cefradine,
minocycline, cefazolin and etc. Artificial regression of healthy M. salmoides infected with A. veronii showing clinical signs
similar to those of diseased fish, which were the same as those of the naturally occurring disease, such as congestion of the
ventral and pectoral fin bases, redness and swelling of the anus, and obvious enlargement of the abdomen, etc. And a large
amount of fluid in the abdominal cavity was found by autopsy.【Conclusion】The above results indicate that A. veronii is the
pathogenic bacterium causing this disease of M. salmoides, and the approved fishery drugs such as neomycin and doxycycline
can be used to control the disease during the breeding process |
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