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| Effect of Bacillus safensis GL2 on the Neutral Aroma Components of Cigar Tobacco Leaves |
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| DOI：10.16768/j.issn.1004-874X.2024.07.010 |
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| Abstract: |
| 【Objective】The use of artificial inoculation to ferment cigar tobacco leaves is an inevitable trend in the
industrial production of cigar tobacco leaves, and the neutral aroma components are the important quality components of cigar
tobacco leaves. The study aims to investigate the effect of Bacillus safensis GL2 on the neutral aroma components of cigar
tobacco leaves.【Method】GL2 was screened from Cuban cigar Cohiba Robert and used to ferment cigar leaves. The neutral aroma components in tobacco leaves were determined by distillation extraction-gas chromatography-mass spectrometry, and
multivariate statistical methods were used for analysis and statistics.【Result】The total aroma and the contents of most neutral
aroma components in cigar tobacco leaves were significantly increased after GL2 fermentation (P<0.05), and the increased aroma
substances mainly included olefins, ketones and alcohols. A total of 13 differentially neutral aroma components were selected by
VIP>1 and P<0.05, and 9 of them were significantly up-regulated and positively correlated with tobacco leaf aroma. There were
7 species with significant positive correlation (P<0.05), which were the key differential aroma components of 2,6,6-trimethyl-2-
cyclohexene-1,4-dione, 2,6,6-trimethyl-1,4-cyclohexanedione, geraniyl acetone, acanthyl acetone, neophydiene, solanone and
phytoalcohol. In addition, 4 different aroma components were significantly decreased and negatively correlated with tobacco leaf
aroma, among which 2,4-di-tert-butyl-6-nitrophenol was significantly negatively correlated with cigar tobacco aroma (P<0.05).
2,6,6-trimethyl-2-cyclohexene-1,4-dione, geranyl acetone, solanone and phytol contributed significantly to the aroma of
fermented cigar tobacco leaves, resulting in a stronger overall dry and floral fragrances. As a result, they enriched the aroma and
improved the sensory quality of cigar tobacco leaves.【Conclusion】Inoculation of B. safensis during cigar tobacco fermentation
is beneficial for improving the quality of cigar tobacco leaves, which provide theoretical support for improving the fermentation
quality of cigar tobacco leaves with biotechnology. |
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