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| Genetic Analysis of Major Gene and Polygene Inheritance of Chlorophyll Content in Chieh-qua Pulp |
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| DOI：10.16768/j.issn.1004-874X.2024.09.010 |
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| Abstract: |
| Abstract: 【Objective】A major gene and polygenes genetic analysis on the chlorophyll content in the pulp of cheih-qua
was conducted to study the relationship and genetic mechanism between pulp color and chlorophyll content, with an aim to lay
a foundation for the breeding of high-quality cheih-qua varieties.【Method】In the study, cheih-qua inbred lines J16 (white
pulp) and FJ5 (green pulp) were used to form four generation populations, P1
, P2
, F1
and F2
. The pulp color of the chieh-qua
was observed by naked eye and the chlorophyll content was measured. The genetic model analysis software package was used
to analyze the chlorophyll content of the pulp, and the maximum likelihood function value and AIC value were obtained. Then,the three models with the smallest AIC values were selected as alternate models and the suitability test was carried out. Finally,
the first- and second-order genetic parameters of the optimal genetic model were calculated, and the genetic effect values of
major genes and polygenes were analyzed.【Result】The results showed that chlorophyll content was the key factor in the
formation of green pulp of the chieh-qua. The color and chlorophyll content of the F1
generation were between the parents and
slightly inclined to the white pulp parents (P1
), and the color and chlorophyll content of the F2
generation showed quantitative
characteristics. The optimal genetic model for chlorophyll content in the pulp was MX2-ADI-ADI, that is, 2 pairs of additive -
dominant - epistatic major gene + additive - dominant - epistatic polygene models. In the first-order genetic parameters, the
sum of absolute additive effects (da
+db
) of the two major genes was greater than the sum of absolute dominant effects (ha
+hb
),
and da > db
, indicated that the negative additive effect of the first major gene was dominant. In epistatic genetic effects, gene
additive-additive interactions were the main positive effects. The second-order genetic parameters indicated that the genetic
variance of the major gene was 32.1590, the genetic variance of the polygenes was 1.1540, and the heritability rates of the major
gene and the polygenes were 93.69% and 3.36%, respectively. 【Conclusion】The heritability of major genes for the pulp color
of chieh-qua is much higher than that of polygenes, and the major genes are dominant. Therefore, we should pay attention to
the use of major genes in the process of high-quality breeding of chieh-qua, and we can select in early generations and improve
chieh-qua by combined breeding. |
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