| 吴雅露，王 颖，陈梦涛，应鹏飞，蒋玉蓉，陆国权.彩叶芋组织快速繁殖技术研究[J].广东农业科学,2019,46(3):31-36 |
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彩叶芋组织快速繁殖技术研究 |
| Study on Rapid Propagation Technology for Caladium bicolor Tissue |
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| DOI：10.16768/j.issn.1004-874X.2019.03.005 |
| 中文关键词: 彩叶芋 生长调节剂 快速繁殖 诱导 增殖 |
| 英文关键词: Caladium bicolor growth regulator rapid propagation induction proliferation |
| 基金项目:农业部转基因生物新品种培育重大专项（2011ZX08005-005）；浙江省重大科技专项计划项目（2011C12030）；青海省海西州科技项目（2012-Y01）；浙江农林大学科研创新项目（KX20180014） |
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| 中文摘要: |
| 【目的】研究最适宜彩叶芋组织快速繁殖的激素浓度，为其工厂化生产提供借鉴和帮助。【方法】
以漆斑彩叶芋和红艳彩叶芋为材料，以 MS 为基本培养基，添加不同浓度的 6-BA 和 NAA 进行组培繁殖，对
幼叶外植体的愈伤组织诱导以及愈伤组织增殖分化体系进行优化。【结果】在不同浓度范围内，不同的植物生
长调节剂组合对愈伤组织诱导、增殖分化有不同影响，但均能促使彩叶芋产生愈伤组织，再生出完整植株。炼
苗后移栽至由泥炭土、蛭石和珍珠岩混合的基质中，瓶苗的成活率可达 90% 以上。【结论】MS+6-BA 4.0 mg/
L+NAA 0.5 mg/L 是适合彩叶芋愈伤组织诱导的最佳培养基，启动时间短，产愈率高；MS+6-BA 2.0 mg/L+NAA 0.2
mg/L 是适合彩叶芋分化增殖的最佳培养基，愈伤组织分化率最高，试管苗的生长情况最好。 |
| 英文摘要: |
| 【Objective】The research aimed to study the optimal hormone concentration for the rapid propagation
of caladium bicolor tissue, and to provide reference and help for its factory production.【Method】Young leaves
developing from C. hortulanum ‘Wightii’ and C. hortulanum ‘Postman Joyner’ were used as the explant to make
an optimization experiment of callus induction and callus proliferation differentiation system, by taking tissue culture
and propagation in different formulations of 6-BA and NAA on MS basal medium. 【Result】Different combinations
of plant growth regulators showed different effects on callus induction, differentiation and proliferation within a certain
mass concentration. All the C. bicolors could induce callus and regenerated plants in these different formulations. After
transplanting to the matrix mixed with peat soil, vermiculite and perlite, the survival rate of seedling was more than 90%.
【Conclusion】The results showed that MS+6-BA 4.0 mg/L+NAA 0.5 mg/L was the best culture medium for callus
induction of C. bicolor with short start-up time and high cure rate, while MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was most
effective for differentiation and proliferation with the highest callus differentiation rate and the best growth of test-tube
seedlings. |
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