索邦和西伯利亚百合的离体再生体系建立

崔均涛 1; 刘凤民 2; 张伟丽 3

文章摘要

崔均涛 1 ，刘凤民 2，张伟丽 3.索邦和西伯利亚百合的离体再生体系建立[J].广东农业科学,2019,46(3):51-56

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Establishment of in vitro Regeneration System of Lilium Sorbonne and Siberia

DOI：10.16768/j.issn.1004-874X.2019.03.008

中文关键词: 东方百合受体系统遗传转化组织培养

英文关键词: Lilium Oriental receptor system genetic transformation tissue culture

基金项目:2017 年度广东省本科高校高等教育教学改革项目（285）

作者	单位
崔均涛 1 ，刘凤民 2，张伟丽 3	1. 仲恺农业工程学院园艺园林学院，广东广州 510225；2. 仲恺农业工程学院教学科研基地，广东广州 510225；3. 仲恺农业工程学院农业与生物学院，广东广州 510225

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中文摘要:

【目的】确定索邦（Sorbonne）和西伯利亚（Siberia）百合的离体再生体系适合的诱导培养基、增殖培养基、生根培养基以及最佳转化受体系统。【方法】选用索邦和西伯利亚百合的鳞茎球作为外植体，比较它们在添加不同激素配比的培养基中的生长状况，得出两种百合诱导愈伤组织和不定芽、继代增殖、诱导生根培养基和对卡那霉素敏感的最适条件。【结果】索邦百合最佳诱导培养基是 MS+2,4-D 1.5~2.0 mg/L，西伯利亚百合最佳诱导培养基为 MS+2, 4-D 1.0~1.5 mg/L；两种百合的最佳继代增殖培养基均为 MS+6-BA 0.5 mg/L+NAA 0.2 mg/L：两种百合的最佳生根培养基为 1/2MS+NAA 0.2 mg/L、1/2MS+IBA 0.2 mg/L；筛选转化植株时，质量浓度为 150 mg/L 的卡那霉素足以抑制非转化细胞或细菌的生长。【结论】为东方百合品种种球工厂化生产及遗传转化再生体系充实理论依据。

英文摘要:

【Objective】The suitable induction medium, proliferation medium, rooting medium and the optimal transformation receptor system were determined for the in vitro regeneration systems of Lilium Sorbonne and Siberia.【Method】 The bulbs of Sorbonne and Siberia were used as explants to compare their growth status in the medium with different hormone ratios. And the optimal conditions of callus and adventitious bud induction, subculture proliferation, induction/rooting medium and kanamycin sensitivity were obtained.【Result】The best induction medium for Sorbonne was MS+2,4-D 1.5- 2.0 mg/L, while the best induction medium for Siberia was MS+2,4-D 1.0-1.5 mg/L. The optimal subculture proliferation medium for the two Lilium species was MS+6-BA 0.5 mg/L+NAA 0.2 mg/L. The optimal rooting media for the two Lilium species were 1/2MS+NAA 0.2 mg/L and 1/2MS+IBA 0.2 mg/L, respectively. When screening transformed plants, kanamycin at a mass concentration of 150 mg/L was sufficient to inhibit the growth of non-transformed cells or bacteria.【Conclusion】 The theoretical basis was provided for the industrialized production and genetic transformation and regeneration system of Lilium oriental varieties.

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