| 熊 湖 1
，郑顺林 1，2，黄 强 1
，龚 静 1
，梁衍铃 2，袁继超 1.不同连作方式马铃薯土壤中 3 酚酸测定方法研究[J].广东农业科学,2019,46(4):1-7 |
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不同连作方式马铃薯土壤中 3 酚酸测定方法研究 |
| Study on the Determination Method of Three Kinds of Phenolic Acids in Potato Soil Under Different Continuous Cropping Patterns |
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| DOI：10.16768/j.issn.1004-874X.2019.04.001 |
| 中文关键词: 马铃薯 连作方式 酚酸 高效液相色谱法 |
| 英文关键词: potato continuous cropping pattern phenolic acid high performance liquid chromatography |
| 基金项目:四川省育种攻关及配套项目（2016NYZ0051-5，2016NYZ0032）；国家现代农业产业技术体系四川马铃薯创新团队建设项目 |
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| 中文摘要: |
| 【目的】为建立快速、准确测定马铃薯土壤中 3 种酚酸类化合物（香草酸、对羟基苯甲酸、阿魏
酸）含量的方法，研究不同连作方式（普通连作、玉 / 薯轮作、强化连作）对马铃薯土壤中以上 3 种酚酸的影响。
【方法】采用高效液相色谱法（HPLC），对色谱条件中测定波长、流动相体系、柱温等参数进行优化，并利用
优化后色谱条件对不同连作方式马铃薯土壤中酚酸进行测定。【结果】在用安捷伦 XDB-C18 柱（250 mm×4.6
mm，5 μm）、检测波长为 λ=280 nm、柱温为 35℃、进样量为 20 μL、流动相为 75%A 液（以冰醋酸调节
pH=2.60）∶25%B 液（乙腈）等度洗脱、双泵体积流量 SI=0.8 mL/min 的色谱条件下测定，3 种酚酸在相应的浓
度范围内与峰面积有良好的线性关系，相关系数均大于 0.99，加样回收率在 94.37%~100.46%，重现性实验的相
对标准偏差在均 0.18 以下。检测不同连作方式马铃薯土壤中 3 种酚酸含量，空白对照 3 种酚酸含量整个生育期
前后差异不显著，而 3 种连作方式下 3 种酚酸含量均为成熟期低于前期，其中玉 / 薯轮作土壤中酚酸含量在成熟
期均低于其他连作方式，且与对照差异显著。【结论】优化高效液相色谱法简便、准确、可靠，可以有效测定
马铃薯土壤中这 3 种酚酸含量；玉 / 薯轮作可以减少土壤中这 3 种酚酸含量，缓解酚酸产生的连作障碍。 |
| 英文摘要: |
| 【Objective】 To establish a method for the determination of three phenolic acid compounds (vanillic acid,
p-hydroxybenzoic acid and ferulic acid) in potato soil in a rapid and accurate manner，a study on the influence of different
continuous cropping patterns (common continuous cropping, corn/potato rotation and intensified continuous cropping) on
three phenolic acid compounds in potato soil was conducted. 【Method】 The wavelength, mobile phase system, column
temperature and other parameters were optimized in chromatographic condition by means of high performance liquid chromatography (HPLC)，and the phenolic acids in potato soil under different continuous cropping patterns were determined in
the optimized chromatographic condition. 【Result】 In certain chromatographic condition (Agilent XDB-C18 column: 250×4.6
mm, 5 μm; detection wavelength: λ=280 nm; column temperature:35℃ ; injection volume: 20μL; mobile phase: 75%A liquid
(glacial acetic acid regulation, pH=2.60); 25% B solution (acetonitrile) isocratic elution; dual-pump volume flow rate: SI=0.8
mL/min). there was a good linear relationship between the three phenolic acids (within a reasonable concentration range) and
peak area with the correlation coefficients greater than 0.99, the recovery rates between 94.37% and 100.46%, and the relative
standard deviations of reproducibility experiments below 0.18. The content of the three phenolic acids in potato soil was detected
under different continuous cropping patterns. There was no significant difference among the content of the three phenolic acids
under the blank control before and after the growth period. The content of the three phenolic acids under the three continuous
cropping patterns in the mature stage was lower than that in the early stage. The content of phenolic acid in corn-potato rotation
soil in the mature stage was lower than that in the other two continuous cropping patterns, with significant difference from the
blank control. 【Conclusion】 With the features of simple, accurate and reliable, optimized HPLC can determine the content
of the three phenolic acids in potato soil effectively. The rotation of corn-potato can reduce the content of these three phenolic
acids in soil and alleviate the continuous cropping disorder caused by phenolic acids. |
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