| 朱 静 1，2，洪陈洁 1，2，吴承祯 3，苏少川 4，颜欢欢 4，林 晗 1，2.千年桐内生真菌的分离鉴定及溶磷能力测定[J].广东农业科学,2019,46(6):46-51 |
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千年桐内生真菌的分离鉴定及溶磷能力测定 |
| Isolation and Identification of Endophytic Fungi from Aleurites montana and Determination of Its Phosphate Solubilizing Capacity |
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| DOI：10.16768/j.issn.1004-874X.2019.06.007 |
| 中文关键词: 千年桐 内生真菌 溶磷性 青霉属 嗜热真菌属 |
| 英文关键词: Aleurites montana endophytic fungi phosphorus dissolving characteristics Penicillium Thermomyces |
| 基金项目:国家“十三五”重点研发计划项目（2017YFD0601304）；高校科技创新专项（KFA17056A）；福建农林大学重点项目建设专项（6112C039N） |
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| 中文摘要: |
| 【目的】对千年桐（Aleurites montana）内生真菌进行分离鉴定，初步筛选具有较高溶磷活性的
内生真菌。【方法】从福建省南平市建阳区的外墩采育场、湖桥工区和书坊林场的千年桐根、茎、叶中分离纯
化得到内生真菌。根据菌落在根、茎、叶中出现频率及其直径生长速度筛选优势菌株，进一步对优势菌进行溶
磷能力的定性测定。【结果】共分离出 155 株菌株，其中茎内生真菌 60 株（38.71%）、叶 50 株（32.26%）、
根 45 株（29.03%）。湖桥样地采集的内生真菌数量最多、外墩样地最少。选出 25 株优势菌株，经形态学和
18 SrDNA 分子鉴定分别为毛霉菌属（Mucor sp.）、青霉属（Penicillium sp.）、拟盘多毛孢属（Pestalotiopsis
sp.）、生赤壳属（Bionectria sp.）、木霉属（Trichoderma sp.）、嗜热真菌属（Thermomyces sp.）、链格孢属（Alternaria
sp.）、盾壳霉属（Coniothyrium sp.）和镰刀菌属（Fusarium sp.）。对优势菌株溶磷效果的试验表明，具有较强
溶磷活性的菌株分属青霉属（Penicillium sp.）和嗜热真菌属（Thermomyces sp.）。【结论】千年桐内生真菌数
量在不同地点和不同器官间分布各有不同，初步确定千年桐内生真菌菌属组成，得到具有较强溶磷活性的菌株
分属青霉属（Penicillium sp.）和嗜热真菌属（Thermomyces sp.）。 |
| 英文摘要: |
| 【Objective】 The endophytic fungi from the Aleurites montana was isolated and identified, and the
endophytic fungi with high phosphate-dissolving activity were initially screened.【Method】 Endophytic fungi were
isolated and purified from roots, stems and leaves of Aleurites montana collected from the Waidun breeding ground, Huqiao
work area and the Shufang forest farm in Jianyang District of Nanping City, Fujian Province. Then the dominant strains
were screened according to the frequency of occurrence of colonies in roots, stems and leaves and the growth rate of their diameters, and the qualitative determination of the ability of solubilizing the dominant bacteria was further carried out. 【Result】
A total of 155 strains were isolated, among which, 60 strains of endophytic fungi were isolated from stems (38.71%), 50 strains
from leaves (32.26%), and 45 strains from roots (29.03%). The number of endophytic fungi collected from the Huqiao sample
area was the largest, while that from the Waidun sample area was the least. 25 dominant strains were identified as Mucor sp.,
Penicillium sp., Pestalotiopsis sp., Bionectria sp., Trichoderma sp., Thermomyces sp., Alternaria sp., Coniothyrium sp. and
Fusarium sp. by morphological characteristics and 18 SrDNA molecules identification. Experiments on the effect of phosphorus
solubilization of dominant strains showed that the strains with strong phosphorus solubilizing activity belonged to Penicillium
sp. and Thermomyces sp. 【Conclusion】 The number of endophytic fungi in A. montana varied in different locations and among
different organs. The strains with strong phosphorus solubilizing activity belonged to Penicillium sp. and Thermomyces sp.. |
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