| 董辰韵 1
，常瑶瑶 1
，钟伟强 2，张安强 2，林雅铃 1.两亲性大分子季铵盐对水稻纹枯病菌抑菌活性表征方法研究[J].广东农业科学,2019,46(6):86-92 |
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两亲性大分子季铵盐对水稻纹枯病菌抑菌活性表征方法研究 |
| Characterization of Antibacterial Activity of Amphipathic Macromolecular Quaternary Ammonium Salts Against Rhizoctonia solani |
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| DOI：10.16768/j.issn.1004-874X.2019.06.012 |
| 中文关键词: 两亲性大分子季铵盐 生长速率法 菌丝称重法 水稻纹枯病菌 |
| 英文关键词: amphipathic macromolecular quaternary ammonium salt growth rate method mycelium weighing method Rhizoctonia solani |
| 基金项目:广州市科技计划项目（201704020084，201803020015）；国家自然科学基金（31772202） |
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| 中文摘要: |
| 【目的】为两亲性大分子季铵盐对植物病原真菌的抑菌活性表征方法的选择提供参考。【方法】
分别采用生长速率法和菌丝称重法表征一类新型两亲性大分子季铵盐〔聚二甲基硅氧烷 -b- 聚丙烯酸酯季铵盐
嵌段共聚物，PDMS-b-(PDMAEMA-BC)，简称 Six-Q5〕、亲水性大分子季铵盐（PQD-BC）和小分子季铵盐（BC）
对水稻纹枯病菌（Rhizoctonia solani）的抑菌活性。【结果】生长速率法可用于 PQD-BC 和 BC 的抑菌活性表征，
但不适合 Six-Q5 的抑菌活性表征；菌丝称重法可用于 3 类季铵盐抑菌活性的表征。PDMS 嵌段长度适中的 Si5-Q5 对 R. solani 的 IC50 与 IC90 分别为 12、59 μg/mL，其抑菌活性与小分子季铵盐相当。【结论】对于两亲性大分子
季铵盐，菌丝称重法较生长速率法更适合其抑菌活性的表征。 |
| 英文摘要: |
| 【Objective】This study provided a reference for the selection of characterization methods of amphiphilic
macromolecule quaternary ammonium salts (QAS) on plant pathogenic fungi.【Method】The antifungal activity of
new amphipathic macromolecular QAS copolymers 〔PDMS-b-(PDMAEMA-BC), labeled as Six-Q5〕, water soluble
macromolecule QAS (PQD-BC) and small molecular QAS (benzalkonium chloride, BC) against Rhizoctonia solani were
characterized by growth rate method and mycelium weighing method respectively.【Result】Growth rate method can be
used to characterize the antimicrobial activity of hydrophilic macromolecular QAS (PQD-BC) and small molecular QAS
(BC), but not used to characterize amphiphilic macromolecular QAS. Mycelial weighing method can be used to characterize
the antimicrobial activity of three kinds of QAS. The IC50 and IC90 of Si5-Q5 with moderate length of PDMS block for R.
solani were 12 μg/mL and 59 μg/mL respectively, and its antifungal activity was similar to that of small molecular QAS.
【Conclusion】 For amphipathic macromolecule QAS, mycelial weighing method was more suitable than growth rate method for the characterization of antifungal activity. |
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