| 蔡时可，梅 瑜，顾 艳，谢梅新，王继华.走马胎的离体培养与快速繁殖[J].广东农业科学,2019,46(10):7-12 |
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走马胎的离体培养与快速繁殖 |
| Culture in vitro and Rapid Propagation of Ardisia gigantifolia |
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| DOI：10.16768/j.issn.1004-874X.2019.10.002 |
| 中文关键词: 走马胎 组织培养 快速繁殖 离体培养 |
| 英文关键词: Ardisia gigantifolia tissue culture rapid propagation culture in vitro |
| 基金项目:广东省农业科学院农业科技特派员精准扶贫乡村产业振兴支撑项目（T2018021）；农业农村部华南都市农业重点实验室开放基金项目（016） |
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| 摘要点击次数: 1888 |
| 全文下载次数: 869 |
| 中文摘要: |
| 【目的】走马胎是我国传统药用植物，长期依赖自然采挖，以致野生资源枯竭，急需发展驯化种植。
采用走马胎的茎作为外植体，建立组织培养快速繁殖方法。【方法】采用 70% 酒精和 0.1% 的升汞溶液为消毒液，
设置不同处理时间对外植体进行消毒，得到无菌外植体后接种在含有 6-BA、KT 和 IBA 的改良 MS 培养基进行
丛芽增殖；采用 1/2 改良 MS 培养基作为基础培养基，添加 NAA 和 IBA 不定芽进行生根诱导，再生植株经过炼
苗后，移栽。【结果】走马胎外植体消毒采用 70% 酒精处理 30 s 结合 0.1% 的升汞溶液消毒处理 7~9 min 最合适，
诱导不定芽的增殖培养基配方为改良 MS 培养基 + 6-BA 1.0 mg/L+KT 1 mg/L+IBA 0.05 mg/L，生根培养基配方为
1/2 改良 MS 培养基 + NAA 0.2 mg/L+IBA 0.3 mg/L。【结论】通过对快速繁殖体系中外植体的消毒方法，优化增
殖和生根培养基，建立走马胎的离体培养与快速繁殖方法，为人工种植提供种苗基础。 |
| 英文摘要: |
| 【Objective】 Ardisia gigantifolia is an important traditional Chinese herbal medicine, which has relied
on harvesting from nature for a long time, resulting that resources are almost exhausted. Therefore, it is urgent to domesticate
and plant such medicine. In this study, stems of A. gigantifolia were took as the explants to establish a rapid propagation
method by tissue culture in vitro.【Method】 The explants were sterilized with 70% alcohol and 0.1% mercury chloride
solution for different treatment time. The obtained aseptic explant were inoculated in the modified MS medium containing
6-BA, KT and IBA for cluster buds proliferation. The 1/2 modified MS medium(as basal medium) with NAA and IBA were
used to induce rooting for the adventitious buds. After seedling refining, the regenerated plants were transplanted. 【Result】
The result showed that disinfecting the stems via 70% alcohol treatment for 30 seconds with 0.1% mercuric chloride solution
treatment for 7-9 mins was the best method. The modified MS medium with 1.0 mg/L 6-BA KT and 0.05 mg/L IBA was the
optimal combination for inducing and reproduction. The 1/2 MS medium with 0.2 mg/L NAA and 0.3 mg/L IBA was suitable
for rooting. 【Conclusion】 By optimizing the sterilization method, proliferation and rooting medium of explants in rapid
propagation system, the method for in vitro culture and rapid propagation of A. gigantifolia was established, which provided
seedling foundation for artificial planting. |
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