| 王凤英 1，2 ，肖乾鹏 1
，王 星 2.黑水虻虫卵冷藏保存技术研究[J].广东农业科学,2019,46(10):82-86 |
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黑水虻虫卵冷藏保存技术研究 |
| Research on Cold Storage Technology for Eggs of Hermetia illucens L． |
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| DOI：10.16768/j.issn.1004-874X.2019.10.013 |
| 中文关键词: 黑水虻 虫卵 冷藏保存 孵化率 幼虫生长发育 |
| 英文关键词: Hermetia illucens L. eggs cold storage hatching rate larval growth and development |
| 基金项目: |
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| 中文摘要: |
| 【目的】持续低温或阴雨天气对黑水虻成虫产卵量造成较大影响，在产卵高峰期冷藏多余虫卵用于
卵量低谷期，将有利于规模化标准化生产黑水虻。【方法】研究了不同发育阶段黑水虻虫卵经 12 ℃冷藏不同天
数后的孵化情况，以及原肠期虫卵经 12 ℃分别冷藏 5、10、15 d 后在 32 ℃下孵化生长发育情况。【结果】初产
虫卵不能直接冷藏，12 ℃冷藏 1 d 后转 32℃孵化，死亡率达 100%。胚盘胚带形成期、原肠期、体壁背合期和破
壳期的虫卵在 12 ℃下冷藏 10 d 转置 32 ℃孵化，平均孵化率分别为 94%、94%、27%、0%，体壁背合期和破壳
期虫卵不适宜冷藏。胚盘胚带形成期虫卵冷藏 13 d 后孵化率降至 80% 以下；原肠期虫卵冷藏 15 d 后孵化率降至
80% 以下。冷藏 5、10 d 原肠期虫卵孵化的幼虫生长发育与对照无显著差异；冷藏 15 d 原肠期虫卵存活率低于对照，
百头虫重无显著差异。【结论】在 12 ℃冷藏的原肠期虫卵能保持较高的存活率，幼虫生长发育正常，该结果为
黑水虻的工厂化生产提供理论依据与实践指导。 |
| 英文摘要: |
| 【Objective】The egg production of Hermetia illucens L.is greatly affected by continuous low temperature
or rainy weather. It is helpful to standardize the production of H. illucens by refrigerating the surplus eggs during the peak
spawning period.【Method】In this paper, the egg hatching rate of H. illucens at different developmental stages after being
refrigerated at 12℃ for different days, and the hatching, growth and development of eggs at gastrula stage at 32℃ after
being refrigerated at 12℃ for 5, 10 and 15 days respectively was studied. 【Result】The results showed that the primiparous
eggs could not be refrigerated directly. Refrigerated at 12℃ for 1 day and then incubated at 32℃ , the mortality rate of
eggs was 100%. The eggs at blastoderm and germ band formation stage, gastrula stage, dorsal closure stage and hatching
stage were refrigerated at 12℃ and then incubated at 32℃ . The average hatching rate were 94%, 94%, 27% and 0%,
respectively. Therefore, the eggs at dorsal closure stage and hatching stage were not suitable for cold storage. The hatching
rate of eggs during blastoderm and germ band formation stage was reduced to less than 80% after 13 days of cold storage.
The hatching rate of the eggs during gastrula stage was reduced to less than 80% after 15 days of cold storage. There were
no significant difference in the growth and development between the larvae hatched from the eggs at gastrula stage that
were refrigerated for 5 and 10 days and the larvae of control group. The survival rate of the eggs at gastrula stage which were refrigerated for 15 days was lower than that of the control group. There were no significant difference in the weight of
one hundred larvae. 【Conclusion】The eggs at gastrula stage refrigerated at 12℃ can maintain a high survival rate, and the
larvae can grow and develop normally. The results can be used to provide theoretical basis and practical guidance for industrial
production of H. illucens. |
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