| Caused by African Swine Fever Viruses (ASFV), African Swine Fever (ASF) is an acute infectious
disease with high morbidity and mortality. And domestic and wild boars of all ages could be infected by such disease.
Due to the complexity of ASFV itself and the insufficient understanding of virulence factors and related protective
genes, there is no effective vaccines and targeted drugs. Since the first report in August 2018, ASF has brought great
threat to the green and healthy development of the pig industry in China. The successful control of ASF relies heavily
on fast and reliable laboratory diagnosis, pig isolation and strict biosecurity measures. The current research progress
of swine fever detection technology in Africa is reviewed from the aspects of molecular biology and immunology
detection, among which molecular biology methods include ordinary PCR, fluorescent quantitative PCR, digital PCR,
and so on, and immunology detection methods include red blood cell adsorption reaction, ELISA, immunotomography
test paper, immune imprinting, etc. in order to provide a selective reference for the diagnosis of swine fever in Africa
under different conditionsIn order to provide more options for the diagnosis of ASFV under different conditions,
the current research progress of ASF detection technologies are review from the aspects of molecular biology and
immunology detection, among which molecular biology methods include conventional PCR, fluorescence quantitative
PCR, digital PCR, isothermal amplification, and immunology detection methods include haemadsorption, ELISA,
immunochromatographic strip and immunoblotting, etc. |
