| 孙 尧，孙 鑫，王 雷.毛果杨 RAV/PLC 基因生物信息学分析[J].广东农业科学,2019,46(12):36-41 |
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毛果杨 RAV/PLC 基因生物信息学分析 |
| Bioinformatics Analysis of RAV/PLCGene in Populus trichocarpa |
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| DOI：10.16768/j.issn.1004-874X.2019.12.005 |
| 中文关键词: 毛果杨 RAV/PLC 结构分析 功能预测 |
| 英文关键词: Populus trichocarpa RAV/PLC structure analysis function prediction |
| 基金项目:黑龙江省自然科学基金联合引导项目（LH2019C059） |
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| 中文摘要: |
| 【目的】利用生物信息学手段，对毛果杨（Populus trichocarpa）RAV/PLC 基因编码蛋白质结构
和特征进行预测。【方法】以 PLC（Phospholipases C）为关键字在 Phytozome 数据库搜索毛果杨磷脂酶 C 基因
序列，以 RAV/PLC 基因及其蛋白质序列为研究对象，利用多种生物信息学分析工具对其基因结构及蛋白功能
进行分析。【结果】在毛果杨 PLC 编码基因中发现了一个可编码 RAV 和 PLC 结构域的特殊基因，该基因在
Phytozome 数据库中编号为 Potri.018G109200，ORF 区域长度为 1 650 bp，编码 549 个氨基酸，预测分子量为
62.72338 ku，理论等电点为 9.16；该基因在茎中表达量最高 , 芽中表达量其次之根和叶中表达量较低。其编码
蛋白包含 1 个 AP2 结构域、1 个 B3 结构域和 1 个 PLC-X 结构域，AP2/B3 结构域和 PLC-X 结构域之间可能存
在一个跨膜区；启动子元件分析表明，RAV/PLC 基因可能与光应答及激素应答相关；String 软件预测 RAV/PLC
基因可能与 MYB103、WRKY49、ABI5、DXS2 存在功能关联；KEGG 分析表明，该基因主要与 RAV 转录因子
信号通路有关；同时利用同源建模方法获得了 RAV/PLC 的完整三维模型。【结论】通过对 RAV/PLC 基因结构
和蛋白功能预测，为该基因克隆及参与信号通路分析提供参考。 |
| 英文摘要: |
| 【Objective】The study was conducted to predict protein structure and features of RAV/PLC in Populus
trichocarpa based on bioinformatics analysis.【Method】RAV/PLC gene sequence was obtained from Phytozome database
with PLC (Phospholipases C) as the key word and multiple bioinformatics tools were used to analyze RAV/PLC gene and protein
sequence.【Results】A 1 650 bp open reading frame (Potri. 018G109200) was identified from Phytozome in P. trichocarpa,
encoding 549 amino acids and a special peptide with a RAV domain and a PLC domain. The predicted molecular weight for
RAV/PLC was 62.72338 ku and the theoretical pI was 9.16. A higher expression of RAV/PLC was observed in stem compared
to leaf, bud and root. An AP2 domain, a B3 domain and a PLC-X domain were contained in RAV/PLC, and there might be a
transmembrane helices structure between the AP2/B3 domain and the PLC-X domain. Promoter analysis showed that RAV/PLC
might mainly be linked to light and hormone response. String delineated the function network between RAV/PLC and MYB103,
WRKY49, ABI5, DXS2; KEGG analysis showed that this gene was mainly related to the RAV transcription factor signaling
pathway. And the complete 3D structure was generated via homology modeling.【Conclusion】The information obtained for RAV/
PLC will provide a foundation for the cloning and signal pathway analysis of RAV/PLC. |
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