| 赵 静 1
，郑丽君 2，张晓东 1
，王连春 1
，万琼莲 1
，王志远 3.三七种植前后土壤营养、酶活性与微生物群落结构的变化研究[J].广东农业科学,2019,46(12):66-73 |
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三七种植前后土壤营养、酶活性与微生物群落结构的变化研究 |
| Study on Changes of Soil Nutrient, Enzyme Activity and Structure of Microbial Community before and after Cropping Panax notoginseng |
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| DOI：10.16768/j.issn.1004-874X.2019.12.009 |
| 中文关键词: 三七 土壤营养 土壤酶活性 微生物群落结构 α 多样性 |
| 英文关键词: Panax notoginseng soil nutrient enzyme activity in soil microbial community structure αdiversity |
| 基金项目:云南省科技厅青年基金（2017FD160） |
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| 摘要点击次数: 1565 |
| 全文下载次数: 558 |
| 中文摘要: |
| 【目的】明确三七连作对土壤养分、酶活性及微生物群落结构的影响，为克服三七连作障碍提供
有效的策略。【方法】结合高通量测序技术，分析种植年限的延长对三七根际土壤真菌群落、细菌群落影响的
相关性。【结果】随着种植年限的延长，土壤 pH 值、有机质、全钾、速效磷、速效钾含量上升，而全氮、全磷、
碱解氮含量下降；土壤酶活性除碱性磷酸酶变化不大外，脲酶、蔗糖酶、多酚氧化酶均明显下降。高通量测序表明，
三七种植前后土壤在科水平上检测分类出占比较高的真菌 15 类、细菌 16 类，其中，对照土壤中真菌主要以
Hygrocybe 为优势菌种，占比为 55.17%；种植三七后土壤中真菌占比降至 0.37%，Fusarium 为优势菌种，占比
为 8.31%，其百分比含量增加 32 倍；种植三七后土壤中的细菌群落组成与种群密度变化明显，Pseudomonas、
Streptomyces 等生防细菌随着三七种植年限的延长呈下降趋势。【结论】随着三七种植年限的延长，土壤微生物
的多样性总体呈上升趋势，土壤酶活性下降，土壤营养失调。 |
| 英文摘要: |
| 【Objective】The study was conducted to clarity the effects of Panax notoginseng continuous cropping
on soil nutrient, enzyme activity and structure of microbial community and provide effective strategy for overcoming the
obstacle to P. notoginseng continuous cropping.【Method】Combined with high-throughput sequencing, the influence
of the extension of planting years on fungi and bacteria community of rhizosphere soil was analyzed.【Result】With the
extension of plant years, the pH value and contents of total potassium, organic matter, available phosphorus and available
potassium in soil were increased, while the contents of total nitrogen, total phosphorus, and alkali-hydrolytic nitrogen weredecreased. Except for alkaline phosphatase, the activities of urease, sucrase and polyphenol oxidase in soil were decreased
obviously. High-throughput sequencing showed that, 15 fungi and 16 bacteria were detected and classified at the family level
in soil before and after planting P. notoginseng. The Hygrocybe among the fungi from the soil（CK）before planting was
the dominant strain, accounting for 55.17%, while the ratio was decreased to 0.37% after planting and the Fusarium was
the dominant strain in the soil, accounting for 8.31%, which was 32 times higher than that before planting. The composition
and population density of bacteria community in soil before and after planting P. notoginseng were obviously different, while
the biocontrol bacteria like Pseudomonas and Streptomyces showed a decreasing trend with the extension of planting years.
【Conclusion】Generally, the microbial diversity in soil showed an increasing trend with the extension of planting years. The
soil enzyme activity descended and the soil nutrition lose balance. |
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