| 【Objective】The effects of different sperm counts on in vitro fertilization and early embryonic development
of mice were studied, and the assisted reproductive technique of mice was further improved by comparative analysis.
【Methods】In the test, sperms obtained from the epididymal tail of 9 weeks old C57BL/6 male mice were randomly selected
and put into HTF culture medium, which cultured in carbon dioxide incubator for 1 hour. The sperm counts of semen solution
of 1, 3, 5, 10, 15 and 20 μL were calculated, the corresponding semen solution absorbed into the oocyte clusters which
obtained after the superovulation of 4 weeks old C57BL/6 female mice, and the in vitro fertilization rate and the development
rate of 2-cell embryos calculated after in vitro culture for 24 hours.【Results】Sperm counts of 1, 3, 5, 10, 15 and 20 μL
semen solution were 0.25 (±0.23)×106, 1.23 (±0.31)×106, 2.03 (±0.35)×106, 3.95 (±0.33)×106, 6.50 (±0.76)×106,
and 9.88 (±0.52)×106 (units/mL). At the same time, the in vitro fertilization rates of mice were 88.92%, 95.17%, 94.29%,
90.56%, 85.89% and 78.26%, respectively, and the development rates of 2-cell embryos were 78.21%, 81.75%, 81.51%,77.42%, 70.47% and 50.76%, respectively. The in vitro fertilization rates and the 2-cell embryo development rates with
sperm counts of 1.23 (±0.31) ×106 and 2.03 (±0.35)×106 (units/mL) were better than those of others, with no significant
difference,but were extremely significantly higher than those with sperm counts of 6.50 (±0.76)×106, 9.88 (±0.52)×106 (units/
mL).【Conclusions】Various sperm counts have significantly different effects on the in vitro fertilization and early embryonic
development of mice. The appropriate sperm numbers were 1.23 (±0.31)×106-2.03 (±0.35)×106 (units/mL) , which can
effectively improve the in vitro fertilization rates of mice and promote the normal development of embryos. |
