| 王立平 1
，何展泳 2，年 海 2，王宏杰 2，马启彬 2.大豆遗传转化方法及再生体系研究进展[J].广东农业科学,2020,47(3):16-27 |
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大豆遗传转化方法及再生体系研究进展 |
| Research Progress in Genetic Transformation and Regeneration System of Soybean |
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| DOI：10.16768/j.issn.1004-874X.2020.03.003 |
| 中文关键词: 转基因大豆 遗传转化 再生体系 转化方法 |
| 英文关键词: genetically modified soybean genetic transformation regeneration system transformation method |
| 基金项目:国家转基因生物新品种培育科技重大专项（2016ZX08004002-007）；农业农村部农产品质量安全监管专项（4100-C17106，21301091702101） |
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| 摘要点击次数: 1455 |
| 全文下载次数: 594 |
| 中文摘要: |
| 转基因技术作为现代生物技术之一，在基因功能研究和转基因育种方面取得重要进展。大豆基因
组测序之后，大豆功能基因组学发展迅速，挖掘控制重要性状的基因用于转基因育种受到广泛关注。随着转基
因大豆新品种培育重大专项的实施，我国建立了基因克隆、遗传转化、功能研究、转基因品系安全评价等转基
因育种研究技术体系和监管体系。其中，转基因大豆遗传转化技术体系的研究取得了较快的进展，主要集中在
高效、稳定遗传转化再生体系的建立和优化，大豆遗传转化方法的探索和优化等方面。对大豆遗传转化体系、
转化方法和转化效率等因素进行阐述，可为大豆基因功能研究和转基因新品种培育相关研究提供参考。 |
| 英文摘要: |
| As one of modern biotechnologies, transgenic technology has made important progress in gene function
research and transgenic breeding. Soybean functional genomics has developed rapidly since the completion of soybean
genome sequencing, and it has attracted wide attentions to excavate genes controlling important characters for transgenic
breeding. With the implementation of the major project of new varieties cultivation of genetically modified soybean, China
has established the research technology system and supervision system of transgenic breeding including gene cloning,
genetic transformation, functional research, safety evaluation of transgenic lines and etc. Among them, the research on
technology system of genetic transformation for transgenic soybean has made rapid progress, which was mainly focused on
the establishment and optimization of stable and efficient regeneration system of genetic transformation, exploration and
optimization of soybean genetic transformation methods and etc. The purpose of this paper is to elaborate the following
aspects including genetic transformation system, transgenic methods and transformation efficiency of soybean and to provide
reference for the researches on gene functions and the cultivation of genetically modified soybean. |
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