文章摘要
徐文刚 1,唐永政 1,张建柏 2,征矢野清 3,邹华锋 4.外源人绒毛膜促性腺激素诱导黑边石斑鱼排卵的研究[J].广东农业科学,2020,47(4):122-128
外源人绒毛膜促性腺激素诱导黑边石斑鱼排卵的研究
Study on the Ovulation of Epinephelus fasciatus Induced by Exogenous Human Chorionic Gonadotrophin Hormone
  
DOI:10.16768/j.issn.1004-874X.2020.04.017
中文关键词: 黑边石斑鱼  HCG 激素  排卵  受精  孵化
英文关键词: Epinephelus fasciatus  HCG hormone  ovulation  fertilization  hatching
基金项目:烟台大学博士科研启动基金(HX2020);日本学术振兴会(JSPS)研究项目(26660173)
作者单位
徐文刚 1,唐永政 1,张建柏 2,征矢野清 3,邹华锋 4 1. 烟台大学海洋学院山东 烟台 2640052. 烟台市海洋经济研究院山东 烟台 2640033. 长崎大学中国东海环境资源研究所日本 长崎 851-22134. 上海海洋大学水产科学国家级实验教学示范中心 /水产种质资源发掘与利用教育部重点实验室上海 201306 
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中文摘要:
      【目的】了解外源人绒毛膜促性腺激素(human chorionic gonadotropin, HCG)诱导黑边石斑鱼(Epinephelus fasciatus)排卵的影响。【方法】以成熟的黑边石斑鱼为研究对象,测量其体长、体质量和肥满度后,在 2018 年 7—8 月,使用 HCG 激素(剂量为 200 IU/kg 或 500 IU/kg)注射分别对 6 尾雌鱼进行成熟诱导。【结果】在 200 IU/kg HCG 处理中,注射 24 h 前有 5 尾雌鱼的卵母细胞处于第三次卵黄球前期阶段;注射 48 h后 2 尾雌鱼出现核移动卵母细胞,1 尾雌鱼的卵母细胞为第三次卵黄球后期阶段;注射 60 h 后 1 尾雌鱼出现排卵现象,其余 5 尾雌鱼均为第三次卵黄球后期阶段。在 200 IU/kg HCG 处理的排卵个体中,注射时其卵母细胞直径为 477.0 µm,注射后 60 h 卵母细胞的直径增加至 624.4 µm。此外,经催产、排卵、受精和孵化后共获得总卵数 16 906 粒,受精率为 68.7%,孵化率为 43.0%。而 500 IU/kg HCG 处理的黑边石斑鱼排卵失败。【结论】使用剂量为 200 IU/kg 的外源 HCG 激素可诱导黑边石斑鱼成熟且排卵,但为了提高排卵率、受精率和孵化率,其注射剂量和效应时间还需要进一步调节
英文摘要:
      【Objective】This study aimed to understand the effects of exogenous human chorionic gonadotropin(HCG)hormone inducing ovulation of the blacktip grouper(Epinephelus fasciatus).【Method】The mature E. fasciatus was used as the target object, and the body length, body weight, and condition factor were measured and calculated. After that, 6 female fishes were injected with HCG(dosage: 200 IU/kg or 500 IU/kg)from July to August in 2018 to conduct maturation induction.【Result】Under the 200 IU/kg HCG treatment, before 24 h of the HCG injection, the tertiary yolk stage 1(Ty1)was detected in oocytes of 5 female fishes. After 48 h of injection, the migratory nucleolus oocytes were found in 2 female fishes, and the tertiary yolk stage 2(Ty2)was detected in oocytes of 1 female fish. After 60 h of injection, the ovulation phenomenon was observed in 1 female fish, and the Ty2 was found in other 5 female fishes. In the ovulation individual of 200 IU/kg HCG treatment, the oocyte diameter was 477.0 μm during the injection while it increased to 624.4 μm after 60 h of injection. Furthermore, after the steps of injection, ovulation, fertilization and hatching, the total number of eggs obtained was 16906, and the fertilization rate and hatching rate were 68.7% and 43.0%, respectively. However, the ovulation of E. fasciatus was not observed in the 500 IU/kg HCG treatment. 【Conclusion】In conclusion, the 200 IU/kg usage of exogenous HCG hormone treatment may induce the maturation and ovulation in the blacktip grouper. However, to improve the fertilization rate and hatching rate, the injected dosage and effective time should be further regulated.
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