| 杨 易1，陈汉才 1，沈 卓 1，周 轩 1，黎庭耀 1，杜志炜 2，杨丽璇 2，张 艳 1.菜心游离小孢子培养高频胚诱导培养基优化[J].广东农业科学,2020,47(5):43- |
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菜心游离小孢子培养高频胚诱导培养基优化 |
| Optimization of High Frequency Embryo Induction Medium for Isolated Microspore Culture of Chinese Flowering Cabbage |
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| DOI：10.16768/j.issn.1004-874X.2020.05.005 |
| 中文关键词: 菜心 小孢子培养 诱导培养基 优化 出胚率 |
| 英文关键词: Chinese flowering cabbage microspore culture induction medium optimization embryo production rate |
| 基金项目:37 |
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| 摘要点击次数: 1644 |
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| 中文摘要: |
| 【目的】对菜心小孢子培养的诱导培养基进行优化，以提高胚胎诱导率，创制优良 DH 系应用于育种实践。【方法】设置不同蔗糖浓度和激素（6-BA 和 NAA）浓度组合的 NLN 诱导培养基，以 10 份不同基因型的菜心为试材，进行小孢子培养及出胚率分析，筛选最佳诱导培养基。【结果】7 份材料诱导出胚状体，不同基因型间的胚胎诱导率差异显著，说明基因型是影响小孢子形成的主要因素；在无激素处理中，100 g/L 蔗糖浓度 NLN（NLN-10）小孢子胚诱导率最高，出胚材料诱导率在 3.45~30.46 胚 / 皿；NLN 含量减半的 1/2 NLN-10培养基处理出胚率约为 NLN-10 处理的 2/3；130 g/L 蔗糖浓度 NLN 处理出胚率显著降低，最高仅为 14 胚 / 皿，蔗糖浓度为 160 g/L 的 NLN（NLN-16）处理未出现胚状体；在 NLN-10 中分别添加 0.1 mg/L 6-BA 和 NAA，促进出胚效果最佳，出胚率最高的 Rt19006 达 46.93 胚 / 皿，是不添加激素处理的 1.5 倍；当 NLN-10 中 6-BA 和NAA 浓度均达到 0.2 mg/L 时，胚胎生长发育受到抑制。【结论】筛选出可有效促进菜心小孢子出胚的诱导培养基配方，即 100 g/L 蔗糖浓度的 NLN+6-BA 0.10 mg/L +NAA 0.10 mg/L 。 |
| 英文摘要: |
| 【Objective】The high frequency embryo induction medium for free microspore culture of Chinese flowering cabbage were optimized in order to improve embryo induction rate and create excellent DH lines for breeding practice.【Method】The NLN induction medium with different concentrations of sucrose and hormone（6-BA and NAA）were set, and 10 Chinese flowering cabbages with different genotypes were used as the experiment materials to conduct microspore culture and induction rate analysis for screening out the optimal induction medium.【Result】The results showed that a total of 7 genotypes produced microspore embryos, and significant differences in embryo induction rate were observed among different genotypes, indicating that genotype was the main factor affecting the formation of microspore. Sucrose concentration in the NLN induction medium had a great influence on the embryo production rate. In the hormone-free group, NLN（NLN-10）with 100 g/L sucrose had the highest microspore induction rate and the embryo production rates of the 7 genotypes were 3.45 - 30.46 embryos/dish. In the 1/2 NLN-10 culture medium treatment group, the embryo production rate was about 2/3 of that in the NLN-10 treatment group. In the NLN-13 treatment group, the embryo production rate was significantly reduced, with the highest rate of 14 embryos/dish. When the sucrose concentration reached 160 g/L（NLN-16）, the embryo production was inhibited. When adding 0.1 mg/L 6-BA and NAA to the NLN-10 medium respectively, the optimal embryogenesis could be obtained, with the highest embryo production rate of 46.93 embryos/dish created by “Rt19006”, which was 1.5 times higher than that of hormone-free group. When the concentration of 6-BA and NAA in NLN-10 reached 0.2 mg/L, the growth and development of embryos were inhibited.【Conclusion】An induction medium formula that can effectively promote the embryo production of microspore in Chinese flowering cabbage is screened out, that is: NLN culture medium containing 100 g/L sucrose and 0.1 mg/L 6-BA and NAA. |
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