| 翟艳芳 1，2，岳 锋 1，王选年1.纳米抗体技术及其在食品安全检测中的应用[J].广东农业科学,2020,47(5):118- |
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纳米抗体技术及其在食品安全检测中的应用 |
| Nanobody Technology and Its Application in Food Safety Detection |
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| DOI：10.16768/j.issn.1004-874X.2020.05.015 |
| 中文关键词: 纳米抗体 纳米抗体库 纳米抗体展示技术 安全监测 |
| 英文关键词: Nanobody Nanobody library Nanobody display technology safety monitoring |
| 基金项目:112 |
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| 摘要点击次数: 1487 |
| 全文下载次数: 736 |
| 中文摘要: |
| 骆驼科动物自然产生的仅由重链组成的抗体称为重链抗体，其中由单个可变结构域组成的称为重链抗体可变区（VHH），也被称为纳米抗体（Nb）。从生物学、免疫遗传学再到医学各个领域的潜在应用来说，纳米抗体具有常规抗体无可比拟的优点，可识别常规抗体不能识别的结构以及隐蔽表位。此外，它还具有高稳定性（耐高温、耐酸碱）、易表达等优点。这些优良特性可使其达到常规抗体无法实现的分析或诊断。因而，纳米抗体成为检测食品中非法添加剂、农药残留及农产品污染的理想抗体。主要论述纳米抗体技术以及在食品安全检测方面应用的最新进展和目前所面临挑战，为纳米抗体更好的应用于食品里的细菌和真菌毒素以及农药残留和农产品污染检测提供借鉴。 |
| 英文摘要: |
| Antibodies composed of heavy chains naturally produced by camels are called heavy chain antibodies. Among them, single variable domains are called heavy chain antibody variable regions（VHH）, and also called Nanobodies（Nb）. From the perspectives of biology, immunogenetics, evolution to potential applications in various fields of medicine, Nanobodies have unparalleled advantages over conventional antibodies. They can recognize structures and hidden epitopes that conventional antibodies cannot. In addition, it has the advantages of high stability（high temperature resistance, acid and alkali resistance） and easy expression. These excellent characteristics can make the analysis or diagnosis that conventional antibodies cannot achieve. Therefore, Nanobody has become an ideal antibody for detecting illegal additives, pesticide residues and agricultural product pollution in food. This article mainly discusses the latest development of Nanobody library technology and its application in food safety detection and the current challenges, to provide references for better application of Nanobody in the detection of bacteria, mycotoxins, pesticide residues and agricultural product pollution in food. |
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