| 袁玉婷.PEG 模拟干旱胁迫西藏甘蓝型春油菜芽期根系特征及抗旱性研究[J].广东农业科学,2020,47(7):18-25 |
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PEG 模拟干旱胁迫西藏甘蓝型春油菜芽期根系特征及抗旱性研究 |
| Root Characteristics and Drought Resistance of Tibetan Spring Rapeseed（Brassica napus L.）at Bud Stage Under PEG Simulation Drought Stress |
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| DOI：10.16768/j.issn.1004-874X.2020.07.003 |
| 中文关键词: PEG 溶液 干旱胁迫 甘蓝型春油菜 芽期 抗旱性 根系 西藏 |
| 英文关键词: PEG solution drought stress spring rapeseed（Brassica napus L.） bud stage drought resistance root system Tibet |
| 基金项目:国家油菜产业技术体系建设专项（CARS-12） |
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| 摘要点击次数: 1712 |
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| 中文摘要: |
| 【目的】了解干旱胁迫对西藏甘蓝型春油菜根系形态的影响以及与抗旱性的关系，筛选抗旱性春油菜品种（系）。【方法】以石英砂为基质，采用 15%（W/V）PEG 溶液模拟干旱胁迫研究西藏甘蓝型春油菜芽期根系特征与抗旱性关系，筛选抗旱性春油菜品种（系）。【结果】干旱胁迫对西藏甘蓝型春油菜相对根重及相对根冠比影响较大，对根长影响最小；自育材料与中国内地引进品种材料抗旱性无差异；相关性分析显示，相对活力指数与相对侧根数（r2=0.443*）、相对根长（r2=0.555*）呈极显著正相关；对 28 份春油菜品种（系）聚类分析显示，在欧式距离 11 处可分为 5 类群，A 类抗旱性最强，平均相对侧根数为 69.14、平均相对根长为96.09%、平均相对根重为 52.58%、平均相对根冠比为 63.16%、平均相对活力指数为 0.59。【结论】相对侧根数、相对根长及相对活力指数可以作为甘蓝型春油菜抗旱鉴定指标，其他指标可作为辅助。结合相对活力指数及聚类分析，筛选到 3 份材料（158106-2、164046-2、188013-1）抗旱性最强。 |
| 英文摘要: |
| 【Objective】The study was conducted to understand the effects of drought stress on the root morphology of Tibetan spring rapeseed（Brassica napus L.）and its relationship with drought resistance, and select drought-resistant varieties（lines）of spring rapeseed.【Method】With quartz sand as the substrate, 15%（W/V）PEG solution was used to simulate the drought stress to study the relationship between the root characteristics and drought resistance of Tibetan spring rapeseed at bud stage, and to screen drought-resistant spring rapeseed varieties（lines）.【Results】Drought stress had a greater impact on the relative root weight and relative root-shoot ratio of Tibetan spring rapeseed, and had the least effect on root length. There was no difference in drought resistance between self-breeding materials and those imported from mainland China. Correlation analysis showed that the vitality index indicated an extremely significant positive correlation with relative lateral root number（r2= 0.443*）and relative root length（r2= 0.555*）. Cluster analysis of 28 spring rapeseed varieties（lines）showed that these varieties could be divided into 5 groups at the Euclidean distance of 11, among which, group A had the strongest resistance, with an average relative lateral root number of 69.14, an average relative root length of 96.09%, an average relative root weight of 52.58%, an average relative root-shoot ratio of 63.16%, and an average relative vitality index of 0.59.【Conclusion】The relative lateral root number, relative root length and relative vitality index could be used as drought-resistant identification indicators of spring rapeseed, and other indicators used as auxiliary. In combination of the relative vitality index and cluster analysis, three materials（158106-2, 164046-2, 188013-1）with strongest drought resistance were screened. |
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