| 徐世强 1，梅 瑜 1，曹 阳 2,3，黄志娜 4，蔡时可 1，王继华 1,3.龙脷叶转录组分析及黄酮类生物合成相关基因的挖掘[J].广东农业科学,2020,47(7):36-44 |
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龙脷叶转录组分析及黄酮类生物合成相关基因的挖掘 |
| Analysis of Transcriptome and Exploration of Genes Related to Flavonoid Biosynthesis in Sauropus spatulifolius Beille |
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| DOI：10.16768/j.issn.1004-874X.2020.07.005 |
| 中文关键词: 龙脷叶 高通量测序 单一序列 黄酮类 功能基因 |
| 英文关键词: Sauropus spatulifolius Beille high-throughput sequencing unigene flavonoids functional gene |
| 基金项目:广东省现代农业产业技术体系创新团队建设项目（2019KJ148）；广东省农业科学院科技创新战略专项资金（高水平农科院建设）- 金颖之星（R2019PY-JX003）；农业农村部华南都市农业重点实验室开放基金（016） |
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| 中文摘要: |
| 【目的】获得龙脷叶（Sauropus spatulifolius Beille）转录组学信息特征及黄酮类生物合成通路基因。【方法】采用高通量测序平台 Illumina HiSeqTM 4000 对龙脷叶进行转录组测序，通过 Trinity 软件 de novo 组装获得 unigene，并基于序列同源性对 unigene 进行功能注释和代谢通路分析。【结果】测序数据经过质控后共获得 88 396 692 个高质量的 reads，通过 de novo 组装获得 46 600 个 unigene，N50 长度为 1 441 bp，平均长度 877 bp。其中 34 188 个（73.36%）unigene 在NR、SwissProt、KOG、GO、KEGG 数据库中得到注释。其中 KEGG 数据库注释到 6 902 个 unigene，涉及 132 条代谢通路。在龙脷叶中我们共鉴定到 56 个 unigene 涉及类黄酮生物合成、9 个 unigene 参与黄酮和黄酮醇生物合成，2 个 unigene 参与异黄酮生物合成。同时还鉴定到 1 256 个转录因子（transcription factors, TFs）和 3 842 个植物抗性基因（R 基因）。MISA 分析发现 46 600 个 unigene 包含 3 356个简单重复序列（simple sequence repeats, SSRs）。【结论】利用高通量测序技术和生物信息分析获得了龙脷叶的转录组信息特征，为后期开展功能基因鉴定、解析黄酮类化合物次生代谢途径及其调控机制奠定研究基础。 |
| 英文摘要: |
| 【Objective】The study was conducted to obtain the transcriptomic characteristics and to identify the synthesis pathway genes related to flavonoid biosynthesis in Sauropus spatulifolius Beille.【Method】The high-throughput sequencing platform Illumina HiSeqTM 4000 was used to perform transcriptome sequencing and the unigene was assembled through de novo assembly by Trinity software. Then the functional annotation and metabolic pathway analysis of these unigenes were performed based on sequence homology.【Result】After quality control of sequencing data, a total of 88 396 692 highquality reads were obtained, and 46 600 unigenes were obtained through de novo assembly. The N50 length was 1 441 bp, with an average length of 877 bp. A total of 34 188（73.36%）unigenes were annotated in NR, SwissProt, KOG, GO and KEGG databases. Among them, 6 902 unigenes were annotated in the KEGG database, involving 132 metabolic pathways. It was identified that 56 unigenes were involved in flavonoid biosynthesis, 9 unigenes involved in flavone and flavonol biosynthesis, and 2 unigenes involved in isoflavonoid biosynthesis. 1 256 transcription factors（TFs）and 3 842 plant resistance genes（R genes）were also identified. MISA analysis showed that 46 600 unigenes contained 3 356 simple sequence repeats（SSRs）.【Conclusion】Transcriptome information characteristics of S. spatulifolius Beille were obtained by the high-throughput sequencing technology and biological information analysis, which provided a research basis for future researches to identify functional genes, flavonoid secondary metabolic pathways and their regulatory mechanisms. |
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