羽脉山黄麻丛枝病感病植株内源激素含量水平的变化

万琼莲 1; 王连春 1; 巢凯翔 1; 赵　静 1; 苏　帆 2; 何孟兰 2; 蔡　红 2

文章摘要

万琼莲 1，王连春 1，巢凯翔 1，赵　静 1，苏　帆 2，何孟兰 2，蔡　红 2.羽脉山黄麻丛枝病感病植株内源激素含量水平的变化[J].广东农业科学,2020,47(7):121-127

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Changes of Endogenous Hormones Levels in Infected Plants Associated with Trema levigata Witches' Broom Disease

DOI：10.16768/j.issn.1004-874X.2020.07.015

中文关键词: 羽脉山黄麻植原体激素失衡定性定量液相色谱串联质谱法（LC-MS/MS）

英文关键词: Trema levigata phytoplasma hormone imbalance qualitative and quantitative liquid chromatography - tandem mass spectrometry（LC-MS/MS）

基金项目:云南省教育厅科学研究基金（2017ZZX176，2017ZDX136）

作者	单位
万琼莲 1，王连春 1，巢凯翔 1，赵　静 1，苏　帆 2，何孟兰 2，蔡　红 2	1. 玉溪师范学院化学生物与环境学院，云南玉溪 653100 2. 云南农业大学农业生物多样性与病虫害控制教育部重点实验室，云南昆明 650201

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中文摘要:

【目的】明确植原体诱发的羽脉山黄麻丛枝病发病和无症状枝叶间六大类植物内源激素含量水平的差异，为羽脉山黄麻丛枝病发病机理的研究奠定基础，为该病害的防治提供理论依据。【方法】野外采集位于云南省新平县同一植株上表现为典型丛枝症状的感病样品和形态正常的健康样品，采用液相色谱串联质谱（LC-MS/MS）法定性定量检测样品中六大类（生长素、细胞分裂素、脱落酸、茉莉酸、水杨酸、赤霉素）共 25种植物激素的含量水平。【结果】共检测到 24 种激素，H2JA 在健康和感病样品均未检测到，cZ 在感病样品中未检测到，GA19 只在感病样品中检测到，GA20、GA24、GA53 只在健康样品中检测到。其中 MESA 含量最高，IAA、IBA、ICA、MEJA、JA、JA-ILE、MESA、SA、ABA 等 9 种激素含量在感病样品和健康样品间存在极显著差异，IP 和 tZ 存在显著差异。赤霉素中 GA3、GA19、GA20、GA7、GA9、GA15 含量水平变化较大。感病样品六大类植物激素含量水平总体下降明显，C/A 比值升高。【结论】研究表明感染植原体的羽脉山黄麻丛枝体内植物内源激素含量水平发生了明显变化。该植原体病害的主要症状为丛枝小叶，是各种致病因子综合影响的结果，其中哪些激素水平的失衡为主要致病因子，还需进一步研究。

英文摘要:

【Objective】The differences of six kinds of endogenous hormone contents between the diseased and asymptomatic branches and leaves of Trema levigata Hand.-Mazz. witches' broom disease induced by phytoplasma were determined, which laid a foundation for the research of pathogenesis of T. levigata Hand.-Mazz. witches' broom disease and provided theoretical basis for the prevention and treatment of this disease.【Methods】The infected samples with typical symptoms of witches' broom and healthy samples with normal morphology were collected from the same plant in the field of Xinping County, Yunnan Province. Liquid chromatography-tandem mass spectrometry（LC-MS/MS）was used to qualitatively and quantitatively detect the contents of 25 types of plant hormones in six categories（auxin, cytokinin, abscisic acid, jasmonic acid, salicylic acid and gibberellin）.【Results】A total of 24 hormones were detected, H2JA was not detected in both infected and healthy samples, cZ was not detected in infected samples, GA19 was only detected in infected samples, and GA20, GA24 and GA53 were only detected in healthy samples. The content of MESA was the highest, there were highly significant differences in contents of IAA, IBA, ICA, MEJA, JA, JA-ILE, MESA, SA and ABA, and significant differences in those of IP and tZ between infected samples and healthy samples. The contents of GA3, GA19, GA20, GA7, GA9 and GA15 in gibberellin changed greatly. The content of six kinds of plant hormones in infected samples decreased significantly and the C/A ratio increased.【Conclusion】The study showed that the contents of plant endogenous hormones in phytoplasma-infected tissues of T. levigata Hand.-Mazz witches' broom changed significantly. The main symptom of this phytoplasma disease was the witches' broom, which was often caused by the comprehensive influence of various pathogenic factors. Further studies were needed to determine which hormone imbalance was the main causative factor.

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