| 姜洪雪，姚丹丹，冯志勇.两种助剂对溴敌隆毒饵的增效作用研究[J].广东农业科学,2020,47(8):103-109 |
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两种助剂对溴敌隆毒饵的增效作用研究 |
| Study on the Synergism of Two Adjuvants on Bromadiolone Baits |
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| DOI：10.16768/j.issn.1004-874X.2020.08.014 |
| 中文关键词: N, N- 二甲基甲酰胺 二甲基亚砜 溴敌隆 毒饵 小白鼠 摄食系数 毒杀效果 |
| 英文关键词: N, N-dimethylformamide dimethyl sulfoxide bromadiolone poison baits mouse feeding coefficient poison effect |
| 基金项目:广东省现代农业产业技术体系创新团队项目（2019KJ113）；广东省农业科学院院长基金（201932）；广东省农业科学院青年导师制项目（R2018QD-063） |
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| 中文摘要: |
| 【目的】为提高杀鼠剂的利用率及防控效果，降低其生态风险，研究了渗透剂对抗凝血灭鼠剂溴敌隆的增效作用。【方法】采用高效液相色谱法，检测添加不同种类及不同浓度的助剂后稻谷毒饵中溴敌隆的含量分布；采用有选择摄食试验和无选择摄食试验的生物测定方法，分别测试不同方法制成的溴敌隆毒谷对小鼠的适口性及毒杀效果。【结果】经 4%N, N- 二甲基甲酰胺及 4% 二甲基亚砜处理后，0.01% 溴敌隆毒谷糙米中的药物含量均显著提高，比常规的 0.01% 溴敌隆毒谷分别增加 271.17% 和 163.51%；而添加 4%N, N- 二甲基甲酰胺和 4% 二甲基亚砜配制的 0.0025% 溴敌隆毒谷，糙米中的药物含量均显著高于常规 0.0025% 溴敌隆毒谷，分别增加 433.33% 和 400.95%；生物测定结果表明，用 4% 的两种助剂配制的 0.0025% 溴敌隆毒谷，对小白鼠均具有较好的适口性及毒杀效果，摄食系数分别为 2.69 和 0.80；毒杀率分别为 100% 和 90%，均显著高于常规的0.0025% 溴敌隆毒谷，与 0.01% 溴敌隆毒谷无显著差异。【结论】使用 4%N, N- 二甲基甲酰胺或 4% 二甲基亚砜作为助剂对溴敌隆毒饵均有显著的增效作用，并可显著降低杀鼠剂的使用浓度。 |
| 英文摘要: |
| 【Objective】In order to increase the utilization rates and prevention and control effects of anticoagulant rodenticides and reduce their ecological risks, the synergistic effects of two adjuvants on bromadiolone baits were investigated.【Method】The content distribution of bromadiolone in the poison grains prepared by different varieties and concentrations of adjuvants were determined by using high-performance liquid chromatography. The palatability and the toxic effects of the bromodiolone poison grains prepared with different adjuvants for mice were evaluated by selective and non-selective feeding tests.【Result】The content of bromadiolone in the brown rice of 0.01% poison grains treated with 4% N, N-dimethylformamide and 4% dimethyl sulfoxide was significantly higher than that in the 0.01% poison grains, with the increase of 271.17% and 163.51%, respectively. While the content of bromadiolone in the brown rice of 0.0025% poison grains treated with 4% N, N-dimethylformamide and 4% dimethyl sulfoxide was significantly higher than that in the 0.0025% poison grains, with the increase of 433.33% and 400.95%, respectively. The results of bioassay showed that the 0.0025% bromodiolone poison grains with two kinds of adjuvants exhibited good palatability and potent efficacy on mice. Their feeding coefficient were 2.69 and 0.80, with the poison rates of 100% and 90%, which were significantly higher than the poison rate of the 0.0025% bromodiolone poison grains, but there was no significant difference with the 0.01% bromodiolone poison grains.【Conclusion】4% N, N-dimethylformamide and 4% dimethyl sulfoxide showed significant synergistic effects to the bromodiolone poison baits, and decreased the use concentration of rodenticide obviously. |
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