| 徐 磊，刘 洋.高粱组Ⅲ WRKY 转录因子对干旱胁迫的表达分析[J].广东农业科学,2021,48(2):11-16 |
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高粱组Ⅲ WRKY 转录因子对干旱胁迫的表达分析 |
| Expression Analysis of Group Ⅲ WRKY Transcription Factors in Sorghum Under Drought Stress |
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| DOI：10.16768/j.issn.1004-874X.2021.02.002 |
| 中文关键词: WRKY 基因 转录因子 高粱 干旱胁迫 PEG 处理 |
| 英文关键词: WRKY gene transcription factor sorghum drought stress PEG treatment |
| 基金项目:中国热带农业科学院基本科研业务费专项资金（1630102017002） |
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| 中文摘要: |
| 【目的】组Ⅲ WRKY 基因在植物应对非生物胁迫中起重要调控作用，分析其家族成员响应干旱胁
迫的表达谱，为该类基因的生物学功能研究提供参考。【方法】利用 NCBI 数据库下载高粱基因芯片数据，分
析组Ⅲ WRKY 基因响应干旱胁迫、热胁迫和复合胁迫的表达谱；以 20% PEG6000 模拟干旱胁迫处理高粱品种
BTx623 幼苗，采用 qRT-PCR 技术检测基因表达谱。【结果】基因芯片分析结果表明，SbWRKY14、SbWRKY32
在干旱胁迫、热胁迫和复合胁迫时下调表达；SbWRKY39 在干旱胁迫、热胁迫时下调表达，但在复合胁迫时上
调表达；SbWRKY41 在 3 种胁迫处理均上调表达。qRT-PCR 检测结果表明，经干旱胁迫处理，SbWRKY10 在处
理后 3、6 h 下调表达，而在处理后 12 h 上调表达；SbWRKY14 在处理后 1、6、12 h 下调表达；SbWRKY16、
SbWRKY38、SbWRKY41 和 SbWRKY42 在处理后 1、3、6、12 h 下调表达；SbWRKY17 在处理后 1、6、12 h 下调
表达；SbWRKY39 和 SbWRKY89 在处理后 1、3、12 h 下调表达；SbWRKY53 在处理后 1、12 h 下调表达，而在
处理后 6 h 上调表达；SbWRKY75 在处理后 1 h 下调表达，而在处理后 3、6、12 h 上调表达；SbWRKY93 在处理
后 6 h 上调表达。【结论】高粱组Ⅲ WRKY 基因可能在高粱应对干旱胁迫中发挥重要作用，不同的 SbWRKY 基
因在应对干旱压力下可能起不同的调控作用。 |
| 英文摘要: |
| 【Objective】Group Ⅲ WRKY genes play an important regulatory role in plant response to abiotic stress.
The research was performed to analyze the expression profiles of their family members in response to drought stress so as to
provide references for the study of biological function of such genes.【Method】Downloading the microarray data from NCBI
database, the expression profiles of group Ⅲ WRKY genes in response to drought, heat and combined stress were analyzed.
Seedlings of sorghum variety BTx623 were treated with 20% PEG6000 under simulated drought stress, the expression
profiles of these genes were detected by qRT-PCR techniques.【Result】Microarray analysis shows that SbWRKY14 and
SbWRKY32 are down-regulated under drought, heat and combined stress. SbWRKY39 is down-regulated under drought and
heat stress, but up-regulated under combined stress. SbWRKY41 is up-regulated under three stress treatments. qRT-PCR
analysis shows that SbWRKY10 is down-regulated at 3 and 6 h, but up-regulated at 12 h. SbWRKY14 is down-regulated at
1, 6 and 12 h. SbWRKY16, SbWRKY38, SbWRKY41 and SbWRKY42 are down-regulated at 1, 3, 6 and 12 h. SbWRKY17 is down-regulated at 1, 6 and 12 h. SbWRKY39 and SbWRKY89 are down-regulated at 1, 3 and 12 h. SbWRKY53 is downregulated at 1 and 12 h while up-regulated at 6 h. The expression of SbWRKY75 is down-regulated at 1 h and up-regulated at 3,
6 and 12 h. SbWRKY93 is up-regulated at 6 h.【Conclusion】These results indicate that the group Ⅲ WRKY genes of sorghum
may play an important role in response to drought stress, and different SbWRKY genes may play different regulatory roles under
drought stress. |
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