NaCl 胁迫西藏青稞芽期幼苗根系生长特性及其耐盐性分析

杨春葆; 原红军

文章摘要

杨春葆，原红军.NaCl 胁迫西藏青稞芽期幼苗根系生长特性及其耐盐性分析[J].广东农业科学,2021,48(2):26-32

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Analysis of Root Growth Characteristics and Salt Resistance of Tibetan Highland Barley at Bud Stage under NaCl Stress

DOI：10.16768/j.issn.1004-874X.2021.02.004

中文关键词: NaCl 溶液青稞根系耐盐性半致死浓度西藏

英文关键词: NaCl concentration highland barley root system salt tolerance median lethal concentration Tibet

基金项目:省部共建青稞和牦牛种质资源与遗传改良国家重点实验室自主课题（XZNKY-2020-C-007Z07）

作者	单位
杨春葆，原红军	省部共建青稞和牦牛种质资源与遗传改良国家重点实验室 / 西藏自治区农牧科学院农业研究所，拉萨西藏 850032

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中文摘要:

【目的】了解 NaCl 胁迫对西藏青稞芽期幼苗根系形态的影响以及与耐盐性的关系，筛选出耐盐性青稞品种（系）。【方法】以石英砂为基质，采用 0、50、100、150、200、250 mmol/L NaCl 溶液处理青稞，以相对活力指数为指标回归分析得到 NaCl 胁迫半致死浓度（LC50），以 LC50 为胁迫浓度处理 28 份青稞，研究 NaCl 胁迫对青稞根系形态特性影响，筛选耐盐性青稞品种（系）。【结果】西藏青稞幼苗相对活力指数随 NaCl 溶液浓度增加呈下降趋势，NaCl 胁迫浓度与相对活力指数回归方程为：y=-0.0022x+0.9107；以 LC50 为浓度胁迫青稞芽期幼苗，发芽率、芽长、最长根长、单株鲜重、单株干重均受到不同程度影响，其中芽长最敏感，单株鲜重最迟钝；相关性分析显示，相对活力指数与相对发芽率显著相关，与最长根长极显著相关，与单株干重极显著正相关；聚类分析结果显示，在欧式距离 3 处可以将青稞分为 4 大类，其中 A 类相对活力指数达到 0.71，耐盐性最强。【结论】NaCl 胁迫青稞半致死浓度为 186.68 mmol/L；发芽率、根长、单株干重可作为青稞耐盐性材料筛选的辅助指标。结合相对活力指数及聚类分析，筛选到 3 份材料（WDM03018、WDM03722、 WDM04526）耐盐性最强。

英文摘要:

【Objective】The study was conducted to understand the influence of NaCl stress on seedling root morphology of Tibetan highland barley at the bud stage and its relationship with salt tolerance, and to screen out salt-tolerant barley varieties（lines）.【Method】With quartz sand as the substrate, the highland barley was treated with 0 mmol/L, 50 mmol/L, 100 mmol/L, 150 mmol/L, 200 mmol/L and 250 mmol/L NaCl solution, and NaCl stress of median lethal concentration（LC50） was obtained by regression analysis with the relative vigor index（RVI）as an indicator. The LC50 of NaCl was used to treat 28 highland barley samples. The effects of NaCl stress on the morphological characteristics of highland barley roots were studied, and salt-tolerant highland barley varieties（lines）were screened out.【Result】The RVI of Tibetan highland barley seedlings showed a decreasing trend with the increase of NaCl solution concentration. The regression equation of NaCl stress concentration and RVI was: y=-0.0022x + 0.9107. With LC50 as the concentration stress, the germination rate, bud length, longest root length, fresh weight per plant and dry weight per plant of highland barley seedlings at the bud stage were all affected to varying degrees. Among them, bud length was the most sensitive and fresh weight per plant was the most insensitive. Correlation analysis showed that the RVI was significantly correlated with the relative germination rate, extremely significantly correlated with the longest root length, and extremely significantly positively correlated with the dry weight per plant. The cluster analysis results showed that, at the third Euclidean distance, the highland barley could be divided into four types, of which the RVI of type A reached 0.71, with the strongest salt tolerance.【Conclusion】The median lethal concentration of highland barley under NaCl stress is 186.68 mmol/L. The germination rate, root length and dry weight per plant can be used as auxiliary indicators for the selection of salttolerant materials for highland barley. Combined with the RVI and cluster analysis, 3 materials（WDM03018, WDM03722, WDM04526）with strongest salt tolerance were screened out.

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