文章摘要
张 云,赵艳菲,王雅平,牟 彬,张嘉越,马浩然,韩玉珠.延薯 4 号马铃薯对氮素的生理生化响应及转录组分析[J].广东农业科学,2021,48(2):56-66
查看全文    HTML 延薯 4 号马铃薯对氮素的生理生化响应及转录组分析
Physiological and Biochemical Response to Nitrogen and Transcriptome Analysis in Yanshu No. 4 Potato
  
DOI:10.16768/j.issn.1004-874X.2021.02.008
中文关键词: 马铃薯  生理响应  转录组分析  氮代谢
英文关键词: potato  physiological response  transcriptome analysis  nitrogen metabolism
基金项目:吉林省产业创新专项资金(201020334)
作者单位
张 云,赵艳菲,王雅平,牟 彬,张嘉越,马浩然,韩玉珠 吉林农业大学园艺学院吉林 长春 130118 
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中文摘要:
      【目的】研究不同氮浓度处理对马铃薯的生理生化响应以及对氮代谢相关基因的挖掘,明确马 铃薯受氮素影响的关键时期以及此时期氮代谢基因的表达差异。【方法】以“延薯 4 号”马铃薯为试材,设置 施氮与未施氮处理,采用盆栽种植的方式研究马铃薯氮效率、生理生化差异以及生长发育关键时期,并且对马 铃薯现蕾期的叶和根进行转录组测序分析,获得氮代谢差异表达基因。【结果】施氮处理显著增加了马铃薯氮 效率、可溶性糖含量、可溶性蛋白含量、根系活力、硝酸还原酶活性、谷氨酰胺合成酶活性,同时现蕾期为马 铃薯生长发育关键时期;施氮的叶和根中共有 12 996 个 DEGs,其中 6 440 个上调,6 556 个下调,GO 富集于 11 个生物过程,17 个细胞成分和 2 个分子功能,在氮代谢途径中共有 15 个 DEGs,8 个上调,7 个下调;未施 氮的叶和根中共有 12 178 个 DEGs,其中 6 268 个上调,5 910 个下调,GO 富集于 7 个生物过程,21 个细胞成 分和 2 个分子功能,在氮代谢途径中共有 19 个 DEGs,8 个上调,11 个下调;氮代谢途径鉴定了编码 9 种基因 的 19 个 DEGs,7 个 DEGs(PGSC0003DMG400016996,PGSC0003DMG400006913,PGSC0003DMG400030212, PGSC0003DMG400025823,PGSC0003DMG400016001,PGSC0003DMG400004355,PGSC0003DMG400009698) 在 叶 片 中 的 表 达 量 较 高, 同 时 有 7 个 DEGs(PGSC0003DMG400015734,PGSC0003DMG400001145, PGSC0003DMG400008262,PGSC0003DMG400008356,PGSC0003DMG400014592,PGSC0003DMG400013235, Novel02273)在根中的表达量较高。【结论】NRT2.4、NRT2.5、NRT2.7、NR、NiR 基因主要参与马铃薯氮素吸 收功能,GdH、GS、GOGAT 基因主要参与马铃薯氮素利用功能。
英文摘要:
      【Objective】The study was conducted to discuss the physiological and biochemical responses of potato treated with different nitrogen concentrations and explore the genes related to nitrogen metabolism, and identify the key period when potato was affected by nitrogen and the expression differences of nitrogen metabolism genes during this period.【Method】Taking Yanshu No. 4 potato as the test material, the nitrogen efficiency, physiological and biochemical differences as well as the key growth and development period of potato were studied by pot planting under nitrogen and non- nitrogen treatments. In addition, transcriptome sequencing was performed on leaves and roots of potato at bud stage to obtain differentially expressed genes of nitrogen metabolism.【Result】Nitrogen treatment significantly increased potato nitrogen efficiency, soluble sugar content, soluble protein content, root activity, nitrate reductase activity and glutamine synthase activity. Meanwhile, the bud stage was a key period of potato growth and development. There were a total of 12 996 DEGs including 6 440 up-regulated and 6 556 down-regulated in leaves and roots treated with nitrogen. GO was enriched in 11 biological processes, 17 cell components and 2 molecular functions, and there were 15 DEGs including 8 up-regulated and 7 down-regulated in nitrogen metabolism pathways. There were a total of 12 178 DEGs in leaves and roots without nitrogen application, including 6 268 were up-regulated and 5 910 were down-regulated. GO was enriched in 7 biological processes, 21 cell components and 2 molecular functions, and there were 19 DEGs including 8 up-regulated and 11 down-regulated in nitrogen metabolism pathways. The nitrogen metabolism pathway identified 19 DEGs encoding 9 genes, in which 7 DEGs (PGSC0003DMG400016996, PGSC0003DMG400006913, PGSC0003DMG400030212, PGSC0003DMG400025823, PGSC0003DMG400016001, PGSC0003DMG400004355, PGSC0003DMG400009698)had high expression in leaves, meanwhile, the other 7 DEGs(PGSC0003DMG400015734, PGSC0003DMG400001145, PGSC0003DMG400008262, PGSC0003DMG400008356, PGSC0003DMG400014592, PGSC0003DMG400013235, Novel02273)had high expression in roots.【Conclusion】NRT2.4, NRT2.5, NRT2.7, NR and NiR genes are mainly involved in nitrogen absorption function of potato, while GdH, GS and GOGAT genes are mainly involved in nitrogen utilization function of potato.
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