文章摘要
徐丹丹 1,2,石力允 2,张 羽 1 ,林泽勉 2,姜子德 2,乔 方 1.广东咖啡炭疽病病原菌初步鉴定及防治药剂筛选[J].广东农业科学,2021,48(2):100-107
查看全文    HTML 广东咖啡炭疽病病原菌初步鉴定及防治药剂筛选
Pathogen Identification of Anthracnose Disease on Coffeaarabica in Guangdong Province and Screening of Fungicides
  
DOI:10.16768/j.issn.1004-874X.2021.02.013
中文关键词: 咖啡  果生炭疽菌  暹罗炭疽菌  鉴定  杀菌剂
英文关键词: Coffea arabica  Colletotrichum fructicola  C. siamense  identification  fungicide
基金项目:深圳职业技术学院博士后启动基金(6019330008K)
作者单位
徐丹丹 1,2,石力允 2,张 羽 1 ,林泽勉 2,姜子德 2,乔 方 1 1. 深圳职业技术学院应用化学与生物技术学院 / 深圳职业技术学院博士后创新实践基地广东 深圳 518055 2. 华南农业大学植物保护学院 / 广东省微生物信号与作物病害防控重点实验室广东 广州 510642 
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中文摘要:
      【目的】明确引起广东咖啡炭疽病的病原菌种类并筛选防治药剂。【方法】采用组织分离法对采 集到的疑似炭疽病咖啡叶片进行分离,单孢纯化后利用柯赫氏法则验证其致病性,结合菌株的形态学特征和多 基因序列(ITS、TUB2、GAPDH、CHS、ACT 和 GS)的系统发育分析对病原菌进行鉴定;利用菌丝生长速率法 测定 4 种常用杀菌剂对病原菌的抑制效果。【结果】分离得到的菌株为果生炭疽菌 Colletotrichum fructicola、暹 罗炭疽菌 C. siamense 和芭蕉生炭疽菌 C. musicola,致病性测定结果显示其均能侵染叶片。咪鲜胺、吡唑醚菌酯 和甲基硫菌灵 3 种杀菌剂对两株强致病性炭疽病菌(果生炭疽菌 C. fructicola CA-13 和暹罗炭疽菌 C. siamense CA-16)的抑制效果最强,其 EC50 值均小于 0.1 mg/L。【结论】广东咖啡炭疽病的优势病原菌为果生炭疽菌 C. fructicola 和暹罗炭疽菌 C. siamense,咪鲜胺、吡唑醚菌酯和甲基硫菌灵可作为防治咖啡炭疽病的首选药剂。
英文摘要:
      【Objective】The study was conducted to clarify the pathogen causing anthracnose disease of Coffea arabica in Guagdong Province, and to screen out control fungicides.【Method】Tissue isolation method was used to isolate and purify the pathogen, and pathogenicity test was conducted by using Koch’s postulates. The pathogen was identified based on morphological characteristics and analysis of phylogenetic sequences(ITS, TUB2, GAPDH, CHS, ACT and GS). Moreover, the inhibitory effects of four common fungicides on the pathogen were measured according to mycelial growth. 【Result】The obtained strains were identified as Colletotrichum fructicola, C. siamense and C. musicola. The results of pathogenicity assay indicated that all the selected isolates could infect leaves. In vitro test on two highly pathogenic strains (C. fructicola CA-13 and C. siamense CA-16)showed that prochloraz, pyraclostrobin and thiophanate-methyl strongly inhibited mycelium growth and their EC50 values were under 0.1 mg/L.【Conclusion】The dominant pathogen causing anthracnose disease on C. arabica leaves were C. fructicola and C. siamense; prochloraz, pyraclostrobin and thiophanatemethyl could be used to control this disease as the first choice.
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