文章摘要
田 力,邱丹缨,温扬敏.蛋白核小球藻对小鼠抗氧化及肠道细菌的影响[J].广东农业科学,2021,48(2):138-145
查看全文    HTML 蛋白核小球藻对小鼠抗氧化及肠道细菌的影响
Effect of Chlorella pyrenoidosa on Antioxidant and Intestinal Bacterial in Mice
  
DOI:10.16768/j.issn.1004-874X.2021.02.018
中文关键词: 小球藻  抗氧化  肠道细菌  拟杆菌属  乳酸杆菌属
英文关键词: Chlorella pyrenoidosa  antioxidant  intestinal bacterial  Bacteroides  Lactobacillus
基金项目:泉州市科技计划项目(2015Z72);泉州市高端海洋人才引进项目(QW201602)
作者单位
田 力,邱丹缨,温扬敏 泉州医学高等专科学校基础医学部福建 泉州 362000 
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中文摘要:
      【目的】研究蛋白核小球藻对小鼠抗氧化活性及肠道细菌的影响。【方法】将 50 只 SPF 小鼠随 机分为正常对照组(等体积生理盐水,CK1)、VE 对照组(100 mg/kg,CK2)和小球藻低剂量、中剂量、高剂 量处理组(100、200、300 mg/kg)。连续灌胃 3 周后,检测小鼠血液和肝组织匀浆总抗氧化能力(T-OAC)和 超氧化物歧化酶(SOD)活性,提取小鼠粪便 DNA、采用高通量测序技术分析小鼠肠道细菌群落结构多样性。 【结果】小球藻低剂量、中剂量、高剂量处理小鼠血液和肝脏组织的 T-OAC 和 SOD 活性均显著高于正常对照。 高通量测序结果显示,小鼠肠道细菌结构以拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)为优势菌门,各 试验组小鼠肠道细菌 Alpha 多样性指数(Shannon、Simpson、Chao1 和 Coverage)与正常对照之间无显著差异。 主成分分析结果显示,小球藻各剂量处理小鼠肠道细菌相似性与正常对照差异显著。与正常对照相比,小球藻 小鼠肠道 Bacteroides 和 Lactobacillus 丰度显著增加,而 Muribaculum 显著减少。【结论】灌胃小球藻能显著增 加小鼠抗氧化能力,提高小鼠肠道有益菌丰度,改善肠道环境。
英文摘要:
      【Objective】The effects of Chlorella pyrenoidosa on antioxidant and intestinal bacterial in mice were analyzed. 【Method】Fifty sparse-fur(SPF)mice were randomly divided into normal group(equal volume of normal saline, CK1), VE group(100 mg/kg, CK2), and different doses of C. pyrenoidosa(100 mg/kg, 200 mg/kg, 300 mg/kg), respectively.After 3 weeks, the total antioxidant capacity(T-AOC)and superoxide dismutase(SOD) activity in serum and liver homogenates of mice were measured. Moreover, the DNA in mouse feces were exacted, and community structure diversity of intestinal bacteria was analyzed by using high-throughput sequencing technology.【Result】It was found that the activity of T-AOC and SOD in serum and liver of mice treated with different doses of C. pyrenoidosa were significantly higher than that of normal group. High-throughput sequencing results showed that the structures of mice intestinal bacteria were dominated by Bacteroides and Firmicutes. Compared with normal group, there was no significantly difference in the Alpha diversity indexes (Shannon, Simpson, Chao1 and Coverage)of intestinal bacteria treated with C. pyrenoidosa. The results of PCA analysis indicated that there was significant difference in intestinal bacteria of mice between treatment with C. pyrenoidosa and normal group. Compared with normal group, the abundance of Bacteroides and Lactobacillus significantly increased in mice treated with C. pyrenoidosa, while the abundance of Muribaculum significantly decreased.【Conclusion】C. pyrenoidosa could increase the antioxidant activity, regulate abundance of beneficial bacteria in intestinal tract, and improve intestinal environment of mice
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