翁志强,许钰滢,吕正阳,刘 娟,王 哲,蔡杨星,王荣波,叶文雨.巨菌草根际土壤微生物群落代谢功能多样性分析[J].广东农业科学,2021,48(4):62-68 |
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巨菌草根际土壤微生物群落代谢功能多样性分析 |
Analysis on Metabolic Functional Diversity of Rhizosphere Microbial Communities of Pennisetum giganteum |
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DOI:10.16768/j.issn.1004-874X.2021.04.009 |
中文关键词: 巨菌草 根际土壤 Biology -ECO 技术 微生物代谢功能多样性 |
英文关键词: Pennisetum giganteum rhizosphere soils Biology-ECO technology metabolic functional diversity of microorganism |
基金项目:福建农林大学科技创新专项基金(KFA19137A,KFA19136A);福建省作物有害生物监测与治理重点实验室开放课题项目(MIMCP-202002) |
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中文摘要: |
【目的】研究福州平潭巨菌草根际土壤与非根际土壤微生物对不同碳源利用,明确巨菌草对根际
土壤微生物功能多样性的影响,为巨菌草生态治理提供理论依据。【方法】在福州平潭基地采集巨菌草(Pennisetum
giganteum)根际土和非根际土壤样品,采用 Biolog-ECO 技术研究巨菌草根际土与非根际土壤微生物对不同碳源
利用特性,揭示不同碳源利用的不同及影响差异的主要因素。【结果】与对照相比,巨菌草根际土壤微生物的
代谢活性高于非根际土壤;多样性分析结果表明,与非根际土壤相比,巨菌草根际土壤样品的 Simpson 指数、
Shannon 指数、Brillouin 多样性指数略高,差异不显著;主成分分析结果表明,对主成分一和主成分二贡献大的
碳源种类分别为 22 种和 6 种,糖类是巨菌草根际土壤微生物利用的主要碳源类型。【结论】与非根际土壤相比,
巨菌草根际土壤微生物群落在碳源利用及代谢功能多样性高于非根际土壤。 |
英文摘要: |
【Objective】The study aimed to explore the utilization of different carbon sources by microbial
communities in rhizophere and non-rhizosphere soil, and identify the influences of rhizophere soil microbial functional
diversities so as to provide a theoretical basis for ecological remediation with Pennisetum giganteum.【Method】The
samples of rhizophere and non-rhizosphere soils of P. giganteum were collected in Pintan Base, Fujian Province. BiologECO method was used to study the characteristics of carbon source utilization by microorganisms in the rhizophere and nonrhizosphere soils of P. giganteum in order to reveal the differences in carbon source utilization and main factors influencing
such differences.【Result】Compared with control, the metabolic activity of microorganisms in the rhizophere soil of P.
giganteum was higher than that in the non-rhizophere soil. The results of diversity analysis showed that, compared with
the non-rhizophere soil samples, the Simpson diversity index, Shannon diversity index and Brillouin diversity index of the rhizophere soil samples were little higher, and no obvious difference was found. Principal component analysis (PCA) showed
that there were 22 and 6 kinds of carbon sources that contributed greatly to PCA 1 and PCA 2, respectively, and carbohydrates
were the main carbon sources for rhizosphere soil of P. giganteum.【Conclusion】The microbial community of P. giganteum
rhizosphere soil has higher carbon source utilization and metabolic diversity than that of non-rhizosphere soil |
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