6-BA 对火凤凰蝴蝶兰成花诱导的影响

赖思婷; 丰 锋; 陈桦洁; 廖 菲; 唐芸妃; 黄朝凤; 陈江萍; 吴日怡

文章摘要

赖思婷，丰锋，陈桦洁，廖菲，唐芸妃，黄朝凤，陈江萍，吴日怡.6-BA 对火凤凰蝴蝶兰成花诱导的影响[J].广东农业科学,2021,48(5):50-56

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Effect of 6-BA on Flowering Induction of‘Huofenghuang’Butterfly Orchid

DOI：10.16768/j.issn.1004-874X.2021.05.007

中文关键词: 蝴蝶兰 6-BA 花芽分化生理生化

英文关键词: butterfly orchid 6-BA flower bud differentiation physiological and biochemical

基金项目:教育部卓越农林人才培养计划项目（GDOU2014041204）；国家级大学生实践教学基地——农科教合作人才培养基地建设专项（GDOU2013040301）；国家级大学生创新创业项目（CXXL2018010、CXXL2020063）

作者	单位
赖思婷，丰锋，陈桦洁，廖菲，唐芸妃，黄朝凤，陈江萍，吴日怡	广东海洋大学滨海农业学院，广东湛江 524088

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中文摘要:

【目的】采用 6-BA 处理蝴蝶兰，研究 6-BA 对蝴蝶兰催花的影响，为蝴蝶兰花期调控提供依据，同时为不易催花品种的成花诱导提供参考。【方法】以火凤凰蝴蝶兰成苗为试验材料，在人工空调催花条件下，采用单因素随机区组试验设计，设置 6-BA 0（CK）、5、10、15、20、25、30 mg/L 等 6 个浓度，每隔 10 d 进行喷施处理，喷施部位为蝴蝶兰自上向下第 2、3、4 叶片节部位，期间测定蝴蝶兰叶片的可溶性糖、可溶性蛋白质含量以及 CAT、POD 活性，调查抽梗率、双梗率、花梗长度、处理到抽梗天数、花朵直径和花蕾数等指标。【结果】25 mg/L 6-BA 处理蝴蝶兰后 30 d 抽梗率为 80.00%，清水对照植株抽梗率仅 11.76%，10~30 mg/L 6-BA 处理后 40 d 抽梗率超过 90.00%、处理后 50 d 全部抽梗；25~30 mg/L 6-BA 处理到抽梗天数为 29 d，显著低于清水处理的 44 d。花芽未抽出时，蝴蝶兰叶片可溶性蛋白质含量最低、可溶性糖含量最高；花芽抽出时，蝴蝶兰叶片 CAT、POD活性逐渐下降。【结论】6-BA 喷施处理蝴蝶兰的最适宜浓度是 25 mg/L，在较短时间内抽梗率达到最高，为低温替代提供了参考依据。

英文摘要:

【Objective】The effect of 6-BA on the flower forcing of butterfly orchid with a view to provide a basis for the regulation of flowering time of butterfly orchid, and to provide a reference for the flowering induction of varieties that are not easy to be induced.【Method】With‘Huofenghuang’butterfly orchid seedlings as test materials, by artificial forcing under the condition of air conditioning, single factor randomized block design was adopted and six 6-BA concentrations including 0（CK）, 5, 10, 15, 20, 25 and 30 mg/L were set up. The 6-BA was sprayed every 10 days, and the spraying position for the butterfly orchid was the 2nd, 3rd and 4th blade section area（from top to bottom）. During the spraying period, the soluble sugar, soluble protein, CAT and POD activity of butterfly orchid leaf were determine, and the indexes such as pedicel extraction rate, double pedicel rate, pedicel length, days from treatment to pedicel extraction flower diameter and flower bud number were investigated.【Result】After being treated with 25 mg/L 6-BA for 30 d, the pedicel extraction, rate of butterfly orchid was 80.00%, while that of water control was only 11.76%. After being treated with 10-30 mg/L 6-BA for 40 d, the pedicel extraction rate of butterfly orchid was more than 90.00%, and all the pedicels were extracted after being treated for 50 d. The time from 25-30 mg/L 6-BA treatment to pedicel extraction was 29 days, which was significantly lower than that of water treatment（44 days）. In accordance with the morphological observation, the soluble protein content in the butterfly orchid leaves was the lowest and the soluble sugar content in the leaves was the highest before the flower buds were extracted. The CAT activity and POD activity of butterfly orchid leaf gradually decreased at the time of flower bud extraction.【Conclusion】The optimal concentration of 6-BA for butterfly orchid was 25 mg/L and the pedicle extraction rate reached the highest in a short period of time, which provided a reference basis for low temperature replacement.

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