| 张小乙，韩 雪，李红雪，王晶莹，王守文，翟璐璐.miR156 介导 SPLs 参与调控拟南芥种子成熟过程[J].广东农业科学,2021,48(8):38-46 |
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miR156 介导 SPLs 参与调控拟南芥种子成熟过程 |
| miR156-mediated SPLs Controls the Regulation of Arabidopsis Seed Maturation |
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| DOI：10.16768/j.issn.1004-874X.2021.08.005 |
| 中文关键词: miR156 SPLs 基因 种子成熟 GUS 表型分析 拟南芥 |
| 英文关键词: microR156 SPLs gene seed maturation GUS phenotype analysis Arabidopsis thaliana |
| 基金项目:国家自然科学基金青年基金（31701895） |
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| 摘要点击次数: 1514 |
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| 中文摘要: |
| 【目 的】MicroRNA156s（miR156s）是一类非常保守的小 RNA 家族，探究其能否参与植物种子
成熟的复杂基因表达调控网络，对提高种子质量和产量等性状具有重要意义。【方法】基于前人建立的报告
基因筛选体系，分别对突变体 MIM156-gus 及 SPLs-ox 进行 GUS 活性分析，利用 qRT-PCR 技术鉴定 miR156
家族不同成员的组织表达特异性及突变体材料中种子成熟关键调控因子的表达情况。【结果】GUS 染色后，
以 gus23 为背景材料的 MIM156 及靶标基因 SPL2、SPL10 和 SPL11 的过表达突变体均在播种后 14 d 的幼苗中
展现出了 GUS 表型。miR156 家族中，miR156a、d、e 和 j 在果荚中的表达量最高，至少是 14 d 幼苗的 20 倍，
且 MIM156 下调表达突变体中 LEC1、ABI3 和 FUS3 的表达水平均有不同程度升高。【结论】miR156 的下调
表达和 SPLs 的过表达诱导 SSPs 基因的异位表达，且 MIM156 促进 LEC1、ABI3 和 FUS3 基因的表达，说明
miR156/SPLs 作为调控因子参与植物种子成熟过程。 |
| 英文摘要: |
| 【 Objective】MicroRNA156s（miR156s）is a kind of very conservative small RNA family. The study
on its participation in the regulatory network of gene expression during seed maturation in plants will play a key role
in the improvement of seed qualities and yields.【Methods】Based on the reporter gene screening system established
by predecessors, the GUS activities of mutants MIM156-gus and SPLs-ox were analyzed. The tissue expression
characteristics of different members in miR156 family and expression of key regulatory factors during mutant seed
maturation process were identified by qRT-PCR technology.【Results】After GUS staining, the four mutants showed
GUS phenotype in 14-day-old seedlings, which were MIM156 and its target genes SPL2, SPL10 and SPL11 with gus23
as background material. In the miR156 family, the expression level of miR156a, d, e and j in silique was at least 20 times
that of in 14-day-old seedlings. The expression levels of LEC1, ABI3 and FUS3 in the MIM156 down-regulated mutants
were significantly increased to different degrees.【Conclusion】The down-regulation of miR156 and the over-expression
of SPLs induced the ectopic expression of SSPs genes. Moreover, MIM156 promoted the expression of LEC1, ABI3 and FUS3 genes. These indicated that miR156/SPLs, as a regulatory factor, was involved in the process of plant seed maturation. |
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