| 胡根海，张晓红，赵元增.越南紫薯胚性愈伤组织诱导及体细胞胚胎形成[J].广东农业科学,2021,48(11):25-31 |
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越南紫薯胚性愈伤组织诱导及体细胞胚胎形成 |
| Embryogenic Callus Induction and Somatic Embryogenesis of Ipomoea batatas from Vietnam |
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| DOI：10.16768/j.issn.1004-874X.2021.11.004 |
| 中文关键词: 越南紫薯 诱导 胚性愈伤组织 体细胞胚胎 再生体系 植株再生 |
| 英文关键词: purple sweet potato from Vietnam induction embryogenic callus somatic embryo regeneration system plant regeneration |
| 基金项目:河南省科技攻关农业领域项目（202102110035） |
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| 中文摘要: |
| 【目的】越南紫薯〔Ipomoea batatas（L.）Lam〕品质优良，薯块均匀，商品性好，但产量偏低，产量性状亟待遗传改良，体细胞胚胎发生是快速繁殖和保持种性稳定的基础，对紫薯的遗传改良有重要意义。采用激素处理越南小紫薯外植体，研究越南紫薯的体细胞胚胎再生途径，为通过体细胞无性系变异筛选紫薯高产优质新材料提供新方法。【方法】以市场销售的越南紫薯为材料，以叶片和茎段作外植体，探讨植物生长调节物质种类（6-BA、NAA、2,4-D、ABA）及配比对越南紫薯培养的影响，建立越南紫薯体细胞胚胎发生及再生植株体系。【结果】叶片和茎段诱导愈伤组织培养基相同，难易程度一致，诱导配方为 MS+6-BA 1.0 mg/L + NAA 0.2 mg/L；在普通愈伤组织诱导形成胚性愈伤组织过程中，2,4-D 起重要作用，其他激素起辅助作用，较好的诱导配方为 MS+6-BA 0.1 mg/L + 2,4-D 2.0 mg/L；在胚性愈伤组织诱导产生体细胞胚过程中，ABA 起着重要作用，并进一步发育和分化的适宜的培养基为 MS+6-BA 0.1 mg/L + NAA 0.2 mg/L + ABA 0.5 mg/L。【结论】叶片和茎段愈伤组织诱导最佳培养基为 MS+6-BA 1.0 mg/L+0.2 mg/L NAA，愈伤组织转化为胚性愈伤最佳培养基为 MS+6-BA 0.1 mg/L +2,4-D 2.0 mg/L；胚性愈伤形成体细胞胚胎最佳培养基为 MS+6-BA 0.1 mg/L + NAA 0.2 mg/L + ABA 0.5 mg/L。 |
| 英文摘要: |
| 【Objective】Purple sweet potato〔Ipomoea Batatas（L.）Lam〕from Vietnam is of excellent quality with uniform tuber and good commodity but low yield and urgently needs genetic improvement of yield traits. Somatic embryogenesis is the basis of rapid reproduction and stable sex, which is of great significance to the genetic improvement of purple sweet potato. The explants of Vietnam small purple sweet potato were treated with hormones to study the somatic embryo regeneration pathway. The experiment provided a new method for screening new materials with high yield and good quality of purple sweet potato by somaclonal variation.【Method】Purple sweet potato from Vietnam was used as the material, and the leaf and stem segments were used as explants. The effects of plant growth regulation substance types（6-BA, NAA, 2,4-D and ABA）and ratios on the purple sweet potato from Vietnam were discussed. Somatic embryogenesis and plant regeneration system for purple sweet potato from Vietnam were established.【Result】The leaf and stem segments induced callus culture mediau were the same and the degree of difficulty was consistent. The induction formula was MS + 1.0 mg/L 6-BA + 0.2 mg/L NAA. The normal callus was induced to form embryonal callus and 2,4-D played an important role, and other hormones played an auxiliary role. The induction formula was MS + 0.1
mg/L 6-BA + 2.0 mg/L 2,4-D. Somatic embryos were induced from embryonic callus and ABA played an important role and suitable medium for further development and differentiation was MS + 0.1 mg/L 6-BA + 0.2 mg/L NAA +0.5 mg/L ABA.【Conclusion】The best induction formula for callus of leaves and stems was MS + 6-BA 1.0 mg/L 6-BA + 0.2 mg/L NAA. The callus was transformed into embryogenic callus by MS + 0.1 mg/L 6-BA + 2.0 mg/L 2,4-D. Somatic embryo formation from embryogenic callus was treated with MS + 0.1 mg/L 6-BA + 0.2 mg/L NAA+ 0.5 mg/L ABA. |
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