| 梅 瑜，徐世强，顾 艳，孙铭阳，周 芳，李静宇，张闻婷，王继华.红脚艾蒿的转录组解析[J].广东农业科学,2021,48(12):174-180 |
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红脚艾蒿的转录组解析 |
| Transcriptomics Analysis of Artemisia rubripes Nakai |
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| DOI：10.16768/j.issn.1004-874X.2021.12.019 |
| 中文关键词: 红脚艾 高通量测序 转录本 SSR 基因注释 |
| 英文关键词: Artemisia rubripes Nakai high-throughput sequencing transcript functional gene SSR gene annotation |
| 基金项目:肇庆市科技计划项目（2020N13004）；广东省农业科学院中青年学科带头人培养项目（R2019PYJX003）；广东省农业科学院创新基金-产业专项（202148） |
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| 中文摘要: |
| 【目的】利用高通量测序技术解析红脚艾（Artemisia rubripes Nakai）的转录组信息特征。【方
法】通过高通量测序平台Illumina HiSeq 2500对红脚艾进行转录组测序，通过Trinity 软件de novo 组装获得
Unigene，并基于序列同源性对Unigene进行功能注释，得到红脚艾的转录组信息。【结果】测序数据经过质控
后共获得24 126 043条高质量的reads，通过de novo 组装获得173 093个转录本，对组装的转录本去冗余后共获得
85 991个Unigene，平均长度为616.87 bp，N50为925 bp。共有47 216个Unigene在NR、KEGG、COG、KOG、GO数
据库获得功能注释，40 802个Unigene在NR数据库注释，显示红脚艾与向日葵（Helianthus annuus）的单基因匹
配率 最高，16 846个Unigene被KEGG数据库注释到130条代谢途径中，26 171个Unigene被注释到25个KOG功能分
类中，23 203个Unigene被GO注释到生物过程、细胞组成和分子功能三大类51个功能分类，12 810个Unigene被注
释到25个COG功能分类中。【结论】利用高通量测序技术获得了红脚艾转录组信息特征，这些数据将为后期开
展功能基因鉴定、解析化合物次生代谢途径及其调控机制奠定研究基础。 |
| 英文摘要: |
| 【Objective】The transcriptome information characteristics of Artemisia rubripes Nakai were analyzed
by high-throughput sequencing technology.【Method】The transcriptome of A. rubripes Nakai was sequenced by Illumina
HiSeq 2500, and Unigene was assembled by Trinity software de novo. The function of Unigene w as annotated based
on sequence homology, and the genetic information of A. rubripes Nakai transcriptome was obtained.【Result】After
quality control of sequencing data, a total of 24 126 043 high quality reads were obtained, and 173 093 transcripts were
obtained through de novo assembly. A total of 85 991 Unigene were obtained after redundance removing of the assembled
transcripts. The N50 length was 925 bp, with an average length of 616.87 bp. 47 216 Unigenes were annotated in NR,
KEGG, COG, KOG and GO databases. Among them, 40 802 Unigenes were annotated in NR, the results showed that the
single gene matching rate between A. rubripes Nakai and Helianthus annuus was the highest, 16 846 Unigenes were
annotated in the KEGG database, involving 130 metabolic pathways, 26171 Unigenes were annotated into 25 functional categories of KOG database, 23 203 Unigenes were annotated in GO database, which were divided into 51 functional
categories of biological processes, cellular components and molecular functions, and 12 810 Unigenes were annotated in 25
KOG functional categories.【Conclusion】The transcriptome information characteristics of A. rubripes Nakai were obtained
by high-throughput sequencing technology. These data will lay a foundation for the identification of functional genes and the
analysis secondary metabolism pathway and its regulatory mechanism. |
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