| 郑雪文，欧阳嫣惟，潘晓璐，张红娜，杨转英.菠萝 AcMADS41 基因的克隆及其在花发育中的表达分析[J].广东农业科学,2022,49(1):42-50 |
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菠萝 AcMADS41 基因的克隆及其在花发育中的表达分析 |
| Analysis on Cloning of AcMADS14 Gene and Its Expression During Flower Development of Pineapple |
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| DOI：10.16768/j.issn.1004-874X.2022.01.005 |
| 中文关键词: 菠萝 AP3 基因 基因表达 生物信息学 单子叶植物 花柱发育 |
| 英文关键词: pineapple AP3 genes gene expression bioinformatics monocotyledons style development |
| 基金项目:国家重点研发计划项目（2019YFD1002205） |
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| 摘要点击次数: 1540 |
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| 中文摘要: |
| 【目的】AP3基因在多种植物花器官发育中具有重要作用，但在菠萝中关于AP3基因及其功能的研
究尚无相关报道，对其研究可为AcMADS41在菠萝花发育中的功能鉴定提供理论依据。【方法】以菠萝花芽为
材料，克隆到一个与花器官发育有关的AP3基因AcMADS41。通过生物信息学分析方法对其理化性质、蛋白结构
域、进化关系、顺式作用元件等进行分析。【结果】该基因DNA全长序列为5 098 bp，编码区为458 bp，编码155
个氨基酸；含有MIKC型MADS-box家族特有的MADS-BOX结构域和K-BOX结构域。系统进化分析结果显示，菠
萝中AcMADS41与海枣（Phoenix dactylifera）和油棕（Elaeis guineensis）的AP3基因聚在一起，推测AcMADS41
在花芽分化过程中起着与AP3同等重要的功能。AcMADS41主要在花中表达，且在菠萝成花前期不表达，在成花
后期高表达。AcMADS41主要集中在花瓣、雄蕊和花柱中表达，在花柱中的相对表达量最高，推测其在花柱发育
过程中起着关键作用。【结论】本研究为AcMADS41在菠萝花发育中的功能鉴定提供了理论依据，对进一步探究
菠萝成花的分子机制具有指导意义 |
| 英文摘要: |
| 【Objective】AP3 plays an important role in the development of floral organs in many plants，However,
there are no relevant reports on AP3 gene and its function in pineapple. This study can provide a theoretical basis for the
functional identification of AcMADS41 in pineapple flower development. 【Method】An AP3 gene, AcMADS41, which may
be involved in flower development, was cloned from pineapple flower bud. The physicochemical properties, protein domains,
evolutionary relationships and cis-acting elements etc. were predicted by bioinformatics analysis.【Result】The full-length
DNA sequence of this gene was 5 098 bp, with a coding region of 458 bp, encoding 155 amino acids. It contained the MADSBOX domain and K-BOX domain unique to the MIKC subfamily of the MADS- box family. Phylogenetic analysis showed
that AcMADS41 clustered with date palm（Phoenix dactylifera）and oil palm（Elaeis guineensis）, and it was speculated
that AcMADS41 would play the same important function as AP3 in flower bud differentiation. Genomic data analysis
showed that AcMADS41 was mainly expressed in flowers. RNA-Seq analysis showed that AcMADS41 was not expressed in early anthesis, but was highly expressed in late anthesis, and was mainly expressed in petals, stamens and styles. The relative
expression of ACMADS41 gene was the highest in styles, which may be a key gene in style development.【Conclusion】The
results provides a theoretical basis for the identification of the function of AcMADS41 in pineapple flower development, which is
of great significance to further explore the molecular mechanism of flower formation in pineapple. |
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