| 曾倩倩，刘 岩，朱 墨，邱宗.基于转录组测序挖掘小麦叶片抗旱相关基因[J].广东农业科学,2022,49(2):1-8 |
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基于转录组测序挖掘小麦叶片抗旱相关基因 |
| Screening of Candidate Genes Resistant to Drought Stressin Wheat Leaves Based on Transcriptome Sequencing |
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| DOI：10.16768/j.issn.1004-874X.2022.02.001 |
| 中文关键词: 小麦 干旱胁迫 RNA-Seq 技术 GO 功能富集 KEGG 代谢通路 |
| 英文关键词: wheat drought stress RNA-Seq technology GO function enrichment KEGG metabolic pathway |
| 基金项目:国家自然科学基金（31500499）；河南省高校科技创新人才项目（16HASTIT019） |
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| 中文摘要: |
| 【目的】通过转录组测序分析干旱胁迫的小麦，以期挖掘小麦响应干旱胁迫的关键基因。
【方法】采用高通量 Illumina Hiseq 2500 测序平台对郑麦 366 号正常生长与自然干旱处理进行测序，并对测序数
据进行分析，筛选响应干旱胁迫的关键基因。【结果】两组样品中分别获得 45 471 018、45 133 470 个 clean read
片段，包含 6.82 Gb、6.39 Gb 碱基信息，拼接组装后，得到 188 334 个转录本和 119 588 个 Unigenes。通过筛选
共获得 1 207 个 DEGs，其中 752 个上调，455 个下调。GO 功能富集分析显示，上调的 DEGs 主要富集在水解酶
活性、催化活性、代谢过程、甘油代谢过程、膜和细胞部分等；下调的 DEGs 富集在细胞器、细胞和氧化还原
酶活性的最多。KEGG 途径富集分析发现，DEGs 显著富集于淀粉和蔗糖代谢、卟啉和叶绿素代谢、光合作用 -
天线蛋白、光合作用、苯丙烷生物合成、乙醛酸和二羧酸代谢、甘油磷脂代谢、氨基酸代谢和光合生物中的碳
固定等代谢通路。5 个抗氧化酶基因的 qRT-PCR 结果与 RNA-Seq 测序结果变化趋势相一致。【结论】利用转录
组测序技术获得了大量小麦抗旱相关基因，为深入研究小麦响应干旱胁迫的分子机制提供更多的基因遗传资源。 |
| 英文摘要: |
| 【Objective】The study was carried out to identify key genes in response to drought stress in wheat
through transcriptome sequencing. 【Method】By using Illumina HiSeq 2500 sequencing platform, sequencing was
employed to Zhengmai 366 exposed to the control and natural drought stress, and the sequencing data were analyzed.
【Result】45 471 018 and 45 133 470 clean reads were obtained from the two sets of samples, containing 6.82 Gb
and 6.39 Gb base information, respectively. After splicing and assembly, 188 334 transcripts and 119 588 Unigenes
were obtained. A total of 1 207 DEGs were screened, of which 752 were up-regulated and 455 were down-regulated.
GO functional enrichment analysis showed that the up-regulated DEGs were mainly enriched in hydrolase activity,
catalytic activity, metabolic process, glycerol metabolism, membrane and cell parts, etc.; however, down-regulated
DEGs were mostly enriched in organelles, cells and oxidoreductase activities. KEGG pathway enrichment analysis
showed that DEGs were significantly enriched in starch and sucrose metabolism, porphyrin and chlorophyll
metabolism, photosynthesis-antenna protein, photosynthesis, phenylpropane biosynthesis, glyoxylic acid and
dicarboxylic acid metabolism, glycerophospholipid metabolism, amino acid metabolism and carbon f ixation in photosynthetic organisms. The qRT-PCR results of 5 antioxidant enzyme genes were consistent with RNA-Seq
sequencing results.【Conclusion】A large number of wheat drought-resistant genes are obtained by transcriptome
sequencing technology, which provides more genetic resources for further study of the molecular mechanism of wheat
response to drought stress. |
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