| 郑 丽，黄杰豪，刘红霞，罗玉明，郭坚华.化学药剂胁迫下 6 株生防菌在黄瓜植株的定殖能力分析[J].广东农业科学,2022,49(2):126-134 |
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化学药剂胁迫下 6 株生防菌在黄瓜植株的定殖能力分析 |
| Analysis of Colonization Ability of Six Biocontrol Bacteria in Cucumber Plants under Chemical Stress Condition |
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| DOI：10.16768/j.issn.1004-874X.2022.02.015 |
| 中文关键词: 生防菌 黄瓜霜霉病 定殖 药剂胁迫 相容性 |
| 英文关键词: biocontrol bacteria cucumber downy mildew colonization fungicide stress compatibility |
| 基金项目:仲恺农业工程学院人才项目 (KA2005408C7)；江苏省环洪泽湖生态农业生物技术重点实验室开放基金（HZHL1006） |
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| 中文摘要: |
| 【目的】探究化学药剂胁迫对生防菌在黄瓜植株上定殖能力的影响。【方法】设置一个温室试验
和两个田间试验，分别在无药剂和无病原菌、无药剂和有病原菌、有药剂和有病原菌的黄瓜植株上处理生防菌。
【结果】供试的 6 株生防菌在黄瓜叶片和根围上均具有良好的定殖效果。在无药剂和无病原菌的温室条件下，
各生防菌定殖量均呈现降 - 增 - 稳定的趋势，菌株 1BS4 和 3BY4 在黄瓜叶片和根围定殖能力较强，接菌后 30 d
定殖量接近 106 CFU/g (FW)，其余菌株定殖量保持在 104 CFU/g(FW) 左右；在无药剂和有病原菌的田间条件下，
各生防菌在叶片和根围的定殖量存在显著差异，且均呈现降 - 增 - 降 - 稳定的趋势，菌株 5BJN1 的定殖能力最强，
接菌后 30 d 在黄瓜叶片和根围定殖量分别为 105.92、104.95 CFU/g(FW)；在药剂和病原菌均存在的田间条件下，各
生防菌在叶片和根围的定殖量存在差异，但差异不显著，也均呈现降 - 增 - 降 - 稳定的趋势，接菌后 30 d 菌株
3BJN7 和 5BJN1 在黄瓜叶片的定殖量较高，分别为 103.88、104.23 CFU/g(FW)，其余菌株定殖量均在 103 CFU/g(FW)
左右。【结论】化学药剂对生防菌定殖能力的影响，取决于菌株类型，如 3BJN7 和 5BJN1 与药剂存在相容性；
在黄瓜霜霉病菌存在时，使用化学药剂后它们仍可保持相当的定殖量；在无药剂胁迫时，霜霉病病原菌的出现
能够诱导菌株 3BJN7 定殖能力的提升。 |
| 英文摘要: |
| 【Objective】The study was carried out to explore the effect of chemical stress on the colonization ability of
biocontrol bacteria on cucumber plants.【Method】By setting a greenhouse experiment and two field experiments, biocontrol
bacteria were inoculated on cucumber plants without fungicides and pathogens, with fungicides and without pathogens, with fungicides and pathogens, respectively.【Result】The six biocontrol strains tested had good colonization effects on cucumber
leaves and roots. Under the greenhouse conditions without fungicides and pathogens, the colonization of each biocontrol strain
showed a trend of decrease-increase-stability, strains 1BS4 and 3BY4 had strong colonization ability in cucumber leaves and
roots. The colonization quantity of strains 1BS4 and 3BY4 was close to 106
CFU/g(FW) at 30 days post inoculation(dpi) about 104
CFU/g(FW); Under the field conditions without fungicides and with pathogens, there were significant differences in the colonization
quantities of various biocontrol strains in leaves and roots, and they all showed a trend of decrease-increase-decrease-stability.
Strain 5BJN1 had the strongest colonization ability. The colonization quantity of cucumber leaves and roots at 30 dpi was 105.92
CFU/g(FW) and 104.95 CFU/g(FW), respectively; Under the field conditions with both fungicides and pathogens, there were
differences in the colonization quantity of biocontrol bacteria in leaves and roots, but the difference was not significant, and all
showed a trend of decrease-increase-decrease-stability, and the colonization quantities of strains 3BJN7 and 5BJN1 in cucumber
leaves at 30 dpi were relatively high, which were 103.88 CFU/g(FW) and 104.23 CFU/g(FW), respectively. The colonization quantity
of other strains was about 103
CFU/g(FW).【Conclusion】The effect of chemicals on the colonization ability of biocontrol bacteria
depends on the types of strains. Some strains (3BJN7 and 5BJN1) have good compatibility with chemicals. In the presence of
cucumber downy mildew, they can still maintain a certain amount of colonization after using chemicals, therefore, they can be
used as potential biocontrol agents; In the absence of fungicide stress, the emergence of downy mildew pathogen can induce the
improvement of colonization ability of strain 3BJN7 so that it can colonize stably on cucumber plants. |
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