| 张 舒，于小航，张 乐，苏 宁，赵丽丽，段 铭.环状 RNA hsa_circ_004389 对甲型流感病毒复制的影响[J].广东农业科学,2022,49(2):135-142 |
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环状 RNA hsa_circ_004389 对甲型流感病毒复制的影响 |
| Effect of Circular RNA hsa_circ_004389 onReplication of Influenza A Virus |
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| DOI：10.16768/j.issn.1004-874X.2022.02.016 |
| 中文关键词: 环状 RNA hsa_circ_004389 甲型流感病毒 宿主 表达调控 |
| 英文关键词: circRNA hsa_circ_004389 influenza A virus host expression regulation |
| 基金项目:国家自然科学基金（31970154） |
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| 中文摘要: |
| 【目的】试验前期组学数据提示甲型流感病毒（Influenza A Virus，IAV）感染 A549 细胞后宿主的
环状 RNA（circular RNA，circRNA）hsa_circ_004389 表达显著上调。为进一步明确 hsa_circ_004389 表达和 IAV
感染间的关联，研究其对 IAV 复制的影响。【方法】（1）采用荧光定量 PCR 等方法，确定在 IAV 病毒感染后
不同时间点和不同感染复数（Multiplicity of Infection，MOI）条件下 hsa_circ_004389 的表达水平；（2）检测聚肌
苷酸胞苷酸（Polyinosinic acid:polycytidilic acid，poly I∶C）和细菌脂多糖（Lipopolysaccharide，LPS）处理后 hsa_
circ_004389 的表达情况；（3）干扰素（Interferon，IFN）β 和 JAK 的抑制剂处理细胞后检测 hsa_circ_004389 的
表达情况；hsa_circ_004389 过表达和敲低后，在 mRNA 和蛋白水平上检测该环状 RNA 对 IAV 复制的影响。【结
果】宿主细胞内 hsa_circ_004389 表达的增加与 IAV 感染相关，且与病毒复制呈明显正相关，而 JAK-STAT 信号
通路参与 hsa_circ_004389 的表达调控，并且 hsa_circ_004389 能够抑制 IAV 复制。【结论】hsa_circ_004389 是宿
主应答 IAV 感染的产物，为潜在的细胞Ⅰ型 IFN 应答 IAV 的拮抗因子。 |
| 英文摘要: |
| 【Objective】Based on the preliminary transcriptome profiles of A549 cells responding to influenza A virus
(IAV) infection, one of the most significantly up-regulated host circular RNA(circRNA), hsa_circ_004389, was selected to
further validate its expression and explore its roles in IAV replication.【Method】First, real-time quantitative PCR (qPCR)
was used to verify the expression levels of hsa_circ_ 004389 at different time courses and different multiplicity of infection
(MOI) of IAV infection in A549 cells; Second, hsa_circ_ 004389 expression in A549 cells treated with poly: I∶C (polyinosinic
acid ∶ polycytidylic acid) and LPS (Lipopolysaccharide) were detected, respectively; Third, A549 cells were treated with
interferon beta (IFNβ) and JAK inhibitor, and the expression of hsa_circ_004389 was determined; Finally, after a transient
overexpression and knockdown in A549 cells, IAV replication at mRNA and protein levels were determined.【Result】
There was a positive correlation between the expression of hsa_circ_004389 and IAV replication. JAK-STAT signaling path
was involved in regulating hsa_circ_004389 expression, and hsa_circ_004389 had the ability to inhibit IAV replication. 【Conclusion】Hsa_circ_004389, the product of the host responding to IAV infection, is a potential host anti-IAV factor. |
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