| 孙莉莉，彭丽娜，李 铮，侯睿宁，牟蕴慧.应用 ISSR 和 SRAP 标记构建李遗传连锁图谱[J].广东农业科学,2022,49(3):40-48 |
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应用 ISSR 和 SRAP 标记构建李遗传连锁图谱 |
| Construction of Genetic Linkage Map of Plum (Prunus salicina L.) with ISSR and SRAP Markers |
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| DOI：10.16768/j.issn.1004-874X.2022.03.005 |
| 中文关键词: 李 遗传图谱构建 分子标记 多态性分析 分离类型 遗传距离 |
| 英文关键词: plum genetic map construction molecular marker polymorphism analysis segregation pattern genetic distance |
| 基金项目:黑龙江省青年科学基金（QC2018027）；黑龙江省博士后资助经费（LBH-Z17203） |
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| 中文摘要: |
| 【目的】李（Prunus salicina L.）是我国东北地区极具经济开发前景的树种，具有重要的社会、经
济价值。通过分子标记技术对李进行遗传连锁图谱构建，为李新品种选育提供理论基础。【方法】以吉林 6 号
和龙园秋李为作图亲本，利用 SSR 引物对 F1 子代进行真实性鉴定，经多态性筛选，选出 14 条 ISSR 引物和 16
对 SRAP 引物用于遗传连锁图谱的构建，应用 Join Map 4.0 软件，结合“双假测交”理论构建李遗传连锁图谱。
【结果】试验获得 72 个真实性 F1 子代，ISSR 引物和 SRAP 引物在 F1 群体产生 5 种分离类型、120 个标记位点，
经软件分析获得 1 张拥有 16 个连锁群的遗传连锁图谱，该图谱总长度为 528.5 cM，包含 38 个分子标记位点，
连锁群平均长度为 13.9 cM，最长的连锁群为 71.1 cM、最短的连锁群为 9.6 cM，多态性标记间最大遗传距离为
52.8 cM、最小遗传距离为 1.4 cM。【结论】研究获得的李遗传连锁图谱丰富了寒地李的遗传研究基础，有助于
李 QTL 定位、分子标记辅助育种和基因定位研究。 |
| 英文摘要: |
| 【Objective】Plum (Prunus salicina L.) is a tree species with great economic prospect in northeast China,
and it has important social and economic values. Genetic linkage maps of plum were constructed by molecular markers to
provide a theoretical basis for breeding new plum varieties.【Method】Jilin 6 and Longyuan Qiuli were used as the mapping
parents. The authenticity of F1
progeny was identified by SSR primers. 14 ISSR and 16 SRAP primers were selected to
construct the genetic linkage map of plum by using Join Map 4.0 software and “double pseudo-testcross” theory.【Result】
In this study, 72 authentic F1
progeny were obtained. ISSR and SRAP primers produced 5 segregation patterns and 120
marker loci in this F1
population. A genetic linkage map with 16 linkage groups and 38 marker loci with a total length of
528.5 cM was constructed by software analysis. The groups ranged in size from 9.6-71.1 cM, and the average size was 13.9
cM. The marker had a maximum genetic distance of 52.8 cM and a minimum genetic distance of 1.4 cM.【Conclusion】The
construction of genetic linkage maps enriched the genetic research basis of plum in cold region, and contributed to the study
of QTL mapping, marker-assisted breeding and gene mapping. |
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