| 徐. 蕾，陈乃明，杨振德，周. 浩，李. 琼，韦善方.寄主转换对云斑白条天牛幼虫肠道细菌多样性的影响[J].广东农业科学,2022,49(4):64-73 |
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寄主转换对云斑白条天牛幼虫肠道细菌多样性的影响 |
| Effects of Host Switching on Diversity of Intestinal Bacteria in Batocera lineolata Larvae |
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| DOI：10.16768/j.issn.1004-874X.2022.04.008 |
| 中文关键词: 云斑白条天牛 寄主转换 可培养细菌 高通量测序 优势菌 肠道细菌多样性 |
| 英文关键词: Batocera lineolata host switching culturable bacteria high-throughput sequencing dominant bacterium diversity of intestinal bacteria |
| 基金项目:广西自然科学基金（2017GXNSFAA198142）；广西林业科技项目（桂林科字〔2014〕第 25 号）；广西高校大学生创新创业训练计划项目（201910593321） |
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| 中文摘要: |
| 【目的】研究寄主转换对云斑白条天牛 Batocera lineolata 幼虫肠道细菌多样性的影响，为从肠道
细菌的角度解析云斑白条天牛的寄主适应性以及开发利用微生物等方面提供理论依据。【方法】以核桃作为原
寄主，桉树作为转换寄主饲养云斑白条天牛幼虫，采用传统微生物分离培养、16Sr RNA 基因测序以及高通量测
序的方法对转换寄主云斑白条天牛幼虫肠道细菌多样性进行研究。【结果】通过传统微生物培养共获得 19 株可
培养细菌，从核桃饲养的云斑白条天牛幼虫肠道中分离出 10 株细菌，转换寄主后，从云斑白条天牛幼虫肠道中
分离出 9 株细菌；两种寄主饲养处理均分离出 6 个属的可培养细菌，其中有 5 个属为共有菌属。高通量测序结
果表明，寄主转换前后的云斑白条天牛幼虫肠道细菌多样性无显著差异。云斑白条天牛幼虫肠道样本中细菌分
别属于 20 个门 32 个纲 55 个目 90 个科 170 个属，其中两种处理云斑白条天牛幼虫肠道优势菌均属于变形菌门
（Proteobacteria）γ- 变形菌纲（Gammaproteobacteria）肠杆菌目（Enterobacteriales）肠杆菌科（Enterobacteriaceae）
肠杆菌科下未确定属（Uncultured bacterium f Enterobacteriaceae）【结论】两种处理云斑白条天牛幼虫肠道细菌
种类组成相似，肠道细菌群落整体多样性比较丰富且菌群较为稳定，短期内改变寄主很难使其肠道细菌多样性
发生改变。 |
| 英文摘要: |
| 【Objective】The study was carried out to investigate the effect of host switching on the diversity
of intestinal bacteria in Batocera lineolata larvae, and find out the host adaptability of B. lineolata larvae from the
perspective of intestinal bacteria, with a view to providing a theoretical basis for the development and utilization of microbes. 【Method】B. lineolata larvae were raised with walnut as primary hosts and Eucalyptus as switching hosts, and the diversity
of intestinal bacteria in B. lineolata larvae was studied by using the methods of traditional microbial culture, 16Sr RNA gene
sequencing and high-throughput sequencing.【Result】A total of 19 culturable strains of bacteria were obtained through
traditional microbial culture. 10 strains of bacteria were isolated from the intestine of the larvae of B. lineolata reared on the
original host, and 9 strains were isolated from the larvae of B. reared by host; 6 genera of culturable bacteria were isolated from
both treatments, 5 of which were common genera. High-throughput sequencing results showed that there were no significant
differences in the diversity of intestinal bacteria in the larvae before and after host switching. B. lineolata larvae intestinal
bacteria samples respectively belonged to 20 phylums, 32 classes, 55 orders, 90 families and 170 genera, including, under two
treatments, both B.lineolata larvae gut dominant fungi belonging to the Proteobacteri, Gammaproteobacteria, Enterobacteriales,
Enterobacteriaceae and Uncultured bacterium f Enterobacteriaceae.【Conclusion】The composition of intestinal bacteria
species of B. lineolata larvae under two treatments was similar, the overall diversity of intestinal bacterial community was
relatively rich and stable, and it was difficult to change the diversity of intestinal bacteria by changing the host in a short period
of time. |
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