| 郑 京，梁乐霞，宣倩倩，郭慧滢，周志国，孙泽敏，左国才.黄瓜疫霉菌拮抗内生细菌的筛选与鉴定[J].广东农业科学,2022,49(4):81-87 |
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黄瓜疫霉菌拮抗内生细菌的筛选与鉴定 |
| Screening and Identification of Antagonistic Endophytic Bacteria Against Phytophthora drechsleri in cucumber |
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| DOI：10.16768/j.issn.1004-874X.2022.04.010 |
| 中文关键词: 内生菌 鉴定 拮抗作用 菌丝抑菌率 掘氏疫霉 |
| 英文关键词: endophyte identification antagonism bacteriostasis rate of mycelium Phytophthora drechsleri |
| 基金项目:河北省自然科学基金（C2020408006）；湖南省自然科学基金（2020JJ7007）；廊坊市科技计划项目（2020013021） |
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| 中文摘要: |
| 【目的】从黄瓜叶片中筛选抗疫霉菌效果好的内生细菌并进行鉴定，为进一步研究黄瓜疫病的理
论探索及防治应用提供实验材料。【方法】以黄瓜叶片为实验材料筛选内生细菌，根据待检菌株的镜检特征、
革兰氏染色反应、菌落排列方式和菌落形态特征等进行初步筛选。采用平板对峙法和发酵液拮抗法实验对目标
内生细菌进行拮抗作用测定。通过 16S rDNA 序列测定及同源性分析，并构建系统发育树确定目标菌株种属。
【结果】初步筛选出 32 株内生细菌将其编号为 CL1~CL32，其中革兰氏阴性杆菌 10 株、革兰氏阳性杆菌 13
株、革兰氏阳性球菌 9 株，利用平板对峙法发现 8 株对掘氏疫霉菌有较强抑制作用的拮抗菌株，菌丝抑制率为
10.3%~54.3%，抑菌半径为 1~7 mm，对其中抑菌效果较好的 3 株菌株（CL7、CL9、CL15）进行发酵液拮抗实验，
结果显示其抑菌率分别为 54.4%、72.2%、63.3%。【结论】对掘氏疫霉菌具有较强抑制作用的 3 株内生细菌（CL7，
CL9、CL15）分别属于泛菌属 Pantoea sp.、假单胞菌属 Pseudomonas sp. 和葡萄球菌属 Staphylococcus sp.，其中
抑制效果最明显的为 CL9，即假单胞菌属 Pseudomonas sp.。 |
| 英文摘要: |
| 【Objective】Endophytic bacteria with good resistance to Phytophthora were screened from cucumber
leaves and identified, so as to provide experimental materials for further study of theoretical exploration and their application
in controlling cucumber blight.【Method】Endophytic bacteria were screened from cucumber leaves, and were preliminarily
screened according to microscopic characteristics, Gram staining reaction, colony arrangement and colony morphology.
The antagonism of endophytic bacteria was tested by plate antagonism method and fermentation liquor antagonism method.
Through 16S rDNA sequencing and homology analysis, phylogenetic tree was constructed to identify the target strains.
【Result】Thirty-two endophytic bacteria were preliminarily screened and numbered as CL1-CL32, including 10 strains of
Gram-negative bacillus, 13 strains of Gram-positive bacillus and 9 strains of Gram-positive cocci. Eight strains were found
to have strong inhibitory effect on Phytophthora drechsleri by plate confrontation method, and the inhibition rate of mycelia ranged from 10.3% to 54.3%. The inhibition radius ranged from 1 mm to 7 mm. Three strains (CL7, CL9, CL15) with better
inhibitory effect were tested in fermentation liquor antagonism experiment, and the inhibition rates were 54.4%, 72.2%, 63.3%,
respectively.【Conclusion】The three endophytic bacteria (CL7,CL9,CL15) with strong inhibitory effect on P. drechsleri
belonged to Pantoea sp., Pseudomonas sp.,and Staphylococcus sp., among which CL9 belonging to Pseudomonas sp. had the
most obvious inhibitory effect. |
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