| 江学斌，成雪鸿，马苗鹏，邓仲晴，李嘉慧.产纤维素酶芽孢杆菌的鉴定与酶活力比较[J].广东农业科学,2022,49(4):116-122 |
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产纤维素酶芽孢杆菌的鉴定与酶活力比较 |
| Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus |
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| DOI：10.16768/j.issn.1004-874X.2022.04.014 |
| 中文关键词: 纤维素酶 纤维素降解菌 筛选 鉴定 芽孢杆菌 |
| 英文关键词: cellulase cellulose degrading bacteria screening identification Bacillus |
| 基金项目: |
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| 摘要点击次数: 1138 |
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| 中文摘要: |
| 【目的】从 14 株细菌中筛选产纤维素酶菌株，为新型饲料添加剂的开发提供材料基础。
【方法】采用刚果红染色法从 14 株细菌中初步筛选出产纤维素酶菌株；对产纤维素酶菌株进行菌落形态观察
和 16S rDNA 基因序列同源性分析鉴定；使用刚果红染色法比较各菌株降解纤维素的能力，用 DNS 法检测各菌
株的纤维素酶活力。【结果】初步筛选出 7 株产纤维素酶菌株，经 16S rDNA 鉴定，其中 4 株为地衣芽孢杆菌、
3 株为枯草芽孢杆菌。刚果红染色法初步判定 7 株芽孢杆菌纤维素降解能力大小表现为 LW006>LW005>LW00
4>LW002>LW007>LW003>LW001，其中菌株 LW006（枯草芽孢杆菌）降解纤维素能力最强，其透明圈直径达
22.5 mm；经 DNS 法测定，7 株芽孢杆菌的纤维素酶活力大小表现为 LW006>LW005>LW004>LW007>LW003>LW
002>LW001，其中菌株 LW006 纤维素酶活力最高，酶活力为 1.22（±0.07）U/mL。【结论】菌株 LW006 是相对
高产纤维素酶的菌株，有望为新型饲料添加剂提供新的菌种资源。 |
| 英文摘要: |
| 【Objective】The research was carried out to screen out celllulase-producing strains from 14 strains of
bacteria in order to provide a material basis for the development of new feed additives. 【Method】The cellulase-producing
strains were screened out from 14 strains by Congo red staining. Then the colony morphologies of them were observed and 16S
rDNA gene sequence homology was analyzed and identified. After comparing the ability of each strain to degrade cellulose by
Congo red staining, the cellulase activity of each strain was detected by DNS method.【Result】Seven cellulase-producing
strains were screened, including 4 strains of Bacillus licheniformis and 3 strains of Bacillus subtilis. Based on Congo red
staining, it was preliminarily identified that the cellulose degradation ability of 7 Bacillus strains was showed as: LW006>LW
005>LW004>LW002>LW007>LW003>LW001. Among them, strain LW006 (B. subtilis) had the strongest ability to degrade
cellulose, producing a clear circle with a diameter of 22.5 mm. The cellulase activities of 7 strains of Bacillus were determined
by DNS method as follows: LW006>LW005>LW004>LW007>LW003>LW002>LW001. Among them, strain LW006
(B. subtilis) showed the highest cellulase activity with 1.22(±0.07) U/mL (P<0.01). 【Conclusion】Strain LW006 is a relatively
high cellulase-producing strain, which is expected to provide new strain resources for new feed additives. |
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